摘要
目的探讨最佳的动脉粥样硬化大鼠模型的制作方法。方法采用单纯高脂饲料饲养、维生素D3结合高脂饲料饲养、维生素D3结合普通高脂饮食饲养3种方法诱导动脉粥样硬化大鼠模型,比较3种方法的模型建立情况。HE染色观察大鼠主动脉血管动脉粥样硬化斑块的组织病理学变化。结果动脉粥样硬化大鼠模型成功情况:单纯高脂饲料喂养大鼠主动脉内膜光滑,未见斑块形成;高脂饲料联合维生素D3组仅2例主动脉内膜形成粟粒样斑块;普通高脂饮食喂养同时加维生素D3灌胃后2个月后可见主动脉内膜明显钙化斑块,呈环状,斑块遍及整条动脉。结论高脂饲料建立动脉粥样硬化模型成模率低,普通高脂饮食饲养联合维生素D3每周间隔灌胃方法诱导动脉粥样硬化大鼠模型成模率高、动物死亡率低,是较好的模型制作方法。
Objective To investigate the optimun approach to induce the atherosclerotic rat model.Methods Three methods were used to induce atherosclerotic model,including： simple high fat diet feeding,high fat diet feeding with gastric perfusion of Vitamin D3,and gastric perfusion of Vitamin D3 with common rat food（splashed oil on the normal rat food with yolks and peanuts）.Histopathology change of the atherosclerotic plaques in aorta was observed by normal and histopathologic sections and Hematoxylin staining.Results The endarterium of aorta of the rats with simple high fat diet was smooth without atherosclerotic plaques after 2 months.2 rats with high fat diet feeding and gastric perfusion of Vitamin D3 had formed miliary atherosclerotic plaques.In the rats with vitamin D3 and common rat food,the ring-shape calcified atherosclerotic plaques were found in the all endarterium of aorta.Conclusion Gastric perfusion of Vitamin D3 once a week（3 times） combined with common rat diet could induce the atherosclerotic model successfully and decrease the mortality rate.
出处
《中国康复理论与实践》
CSCD
2010年第12期1134-1136,F0003,共4页
Chinese Journal of Rehabilitation Theory and Practice
