期刊文献+
任意字段
题名或关键词
题名
关键词
文摘
作者
第一作者
机构
刊名
分类号
参考文献
作者简介
基金资助
栏目信息

大汗腺中分泌物气味结合蛋白、雄激素受体表达水平及其与腋臭相关性研究 被引量：14

Correlation study of apocrine secretion odor-binding protein,expression of androgen receptor and relationship with Osmidrosis
下载PDF
导出
摘要 目的:对腋臭患者和正常人腋部大汗腺进行形态学观察,探讨大汗腺分泌活动的周期。对两种人群大汗腺组织中雄激素受体(androgen receptor,AR)和大汗腺分泌物气味结合蛋白(apocri ne secreti on odor-bi ndi ng protei n,ASOB)的表达进行检测,探讨二者的表达水平及其在腋臭发病中的作用。方法:对腋部皮肤组织进行HE染色,分析大汗腺不同分泌时期的形态和特点。利用免疫组织化学(i mmunohi stochemi stry)和免疫印迹法(eWstern bl otti ng)检测腋臭组与对照组大汗腺组织中AR和ASOB在大汗腺以及不同分泌时期中的表达情况,使用Image-Pro Pl us 5.0和Fl uorChemFC2分析系统对结果进行分析。结果:①大汗腺的分泌活动具有周期性,可分为活跃期和静息期;②腋臭组中AR和ASOB的表达明显高于对照组;③AR和ASOB在大汗腺不同分泌时期的表达差异有统计学意义;④AR和ASOB在两组人群相同分泌时期的表达差异有统计学意义;⑤AR和ASOB在腋臭大汗腺中的表达有正的直线相关关系。结论:AR和ASOB在腋臭患者大汗腺组织中的表达量同时增多,它们可能在腋臭发病的过程中起了重要作用。 Objective To observe the histomorphology of the osmidrosis patients and normal apocrine sweat glands and discuss the active cycle of apocrine sweat gland.Inspection of the expression of androgen receptor（AR） and apocrine secretion odor-binding protein（ASOB）between Osmidrosis and normal people groups,to discuss the effects of two groups on expression level and the application of osmidrosis pathogenesis function.Methods Axilla skin were stained by HE dye,and analyzing the morphologic change and characteristic in different secretion cycle of apocrine sweat glands.The expression of AR and ASOB in two groups and in different secretion cycle were analyzed by immunohistochemistry and Western blotting.Using The analysis system of image-Pro Plus 5.0 and FluorChem FC2 to analyze the results.Results ① Apocrine sweat glands secretion activity had periodicity,and could be classified into active phase and static phase;② The expression of AR and ASOB in osmidrosis was significantly higher than control group.③ The expression of AR and ASOB in different seretion phases of apocrine sweat glands had statistical significance.④ The expression of AR and ASOB in osmidrosis of two groups people was considered statistically significant.⑤ The experession of AR and ASOB in osmidrosis apocrine sweat gland had positive straight line correlation.Conclusion The expression of AR and ASOB in the apocrine sweat glands of osmidrosis was increasing at the same time,they possible play important role in the pathogenesis of human Osmidrosis process.
作者 栗颖利 陈辉 杨国栋 曹彦 杜洁 孙超峰 李晓莉
机构地区 第四军医大学唐都医院烧伤整形科 第四军医大学基础部生物化学教研室
出处 《中国美容医学》 CAS 2010年第12期1822-1826,共5页 Chinese Journal of Aesthetic Medicine
关键词 腋臭 大汗腺 雄激素受体 大汗腺分泌物气味结合蛋白 osmidrosis apocrine sweat glands androgen receptor apocrine secretion odor-binding protein
分类号 R758.74 [医药卫生—皮肤病学与性病学]
  • 相关文献

参考文献13

  • 1Gallagher M,Wysocki CJ,Leyden JJ,et al.Analyses of volatile organic compounds from human skin[J].Bri J Dermatol,2008,159 (4):780-791.
  • 2Spielman AI,Zeng XN,Leyden JJ,et al.Proteinaceous precursors of human axillary odor:isolation of two novel odor-binding proteins[J].Experientia,1995,51 (1):40-47.
  • 3Akutsu T,Sekiguchi K,Ohmori T,et al.Individual comparisons of the levels of (E)-3-methyl-2-hexenoic acid,an axillary odor-related compound,in Japanese[J].Chem Senses,2006,31 (6):557-563.
  • 4Natsch A,Schmid J,Flachsmann F.Identi?cation of odoriferous sulfanylalkanols in human axilla secretions and their formation through cleavage of cysteine precursors by a C-S lyase isolated from axilla bacteria[J].Chem Biodivers,2004,1(7):1058-1072.
  • 5栗颖利,陈辉.腋臭发病机制的研究进展[J].中国美容医学,2010,19(7):1082-1084. 被引量：40
  • 6Kurata S,Itami S,Komada S,et al.Int ranuclear androgen and cytosolic receptor concent rations in the axillary skin of osmidrosis[J].Arch Dermatol Res,1990,282(1):33-37.
  • 7Beier K,Ginez I,Schailer H.Localization of steroid hormone receptors in the apocrine sweat glands of the human axilla[J].Histochemistry and Cell Biology,2005,123(1):61-65.
  • 8Spielman AI,Zeng XN,Leyden JJ,et al.Proteinaceons precursors of human axillary odor:isolation of two novel odor-binding proteins[J].Experientia,1995,51 (1):40-47.
  • 9Zeng XN,Leyden J J,Brand JG,et al.An investigation of human apocrine gland secretion for axiilary odor precursors[J].J Chem Ecol,1992,18(7):1039-1055.
  • 10Natsch A,Gfeller H,Gygax P,et al.A specific bacterial aminoacylase cleaves odorant precursors secretedin the human axiila[J].J Biol Chem,2003,278(8):5718-5727.

二级参考文献23

  • 1Akutsu T,Sekiguchi K, Ohmori T,et al.Individual comparisons of the levels of (E)-3-methyl-2-hexenoic acid, an axillary odor-related compound, in Japanese [J].Chem Senses,2006,31:557-563.
  • 2Natsch A,Schmid J,Flachsmann F.Identi?cation of odoriferous sulfanylalkanols in human axilla secretions and their formation through cleavage of cysteine precursors by a C-S lyase isolated from axilla bacteria[J]. Chem Biodivers,2004,1:1058-1072.
  • 3Leyden JJ,McGinley KJ,Hlozle E,et al.The microbiology of the human axilla and its relationship to axillary odor [J].J Invest Dermatol, 1981,77(5):413 -416.
  • 4Akutsu T,Sekiguchi K, Ohmori T,et al.Individual comparisons of the levels of (E)-3-methyl-2-hexenoic acid, an axillary odor-related compound in Japanese[J].Chem Senses,2006,31 (6):557-563.
  • 5Zeng XN,Leyden JJ,Brand JG,et al.An investigation of human apocrine gland secretion for axillary odor precursors[J].J Chem Ecol, 1992,18(7): 1039-1055.
  • 6Gallagher M, Wysocki CJ, Leyden JJ,et al.Analyses of volatile organic compounds from human skin [J]. Bri J Dermatol,2008,159 (4): 780-791.
  • 7Spielman AI,Zeng XN,Leyden JJ,et al. Proteinaceous precursors of human axillary odor: isolation of two novel odor-binding proteins [J]. Experientia, 1995,51 (1):40-47.
  • 8Natsch A,Gfeller H,Gygax P, et al.Aspecific bacterial aminoacylase cleaves odorant precursors secretedin the human axilla [J].J Biol Chem,2003,278(8):5718-5727.
  • 9Kurata S,Itami S,Komada S,et al.Int ranuclear androgen and cytosolic receptor concent rations in the axillary skin of osmidrosis [J].Arch Dermatol Res,1990,282(1):33-37.
  • 10Beier K,Ginez I,Schaller H.Localization of steroid hormone receptors in the apocrine sweat glands of the human axilla [J]. Histochemi Cell Biol,2005,123(1):61-65.

共引文献39

同被引文献114

引证文献14

二级引证文献48

中国美容医学
2010年 第12期

相关作者

内容加载中请稍等...

相关机构

内容加载中请稍等...

相关主题

内容加载中请稍等...

浏览历史

内容加载中请稍等...
;
使用帮助 返回顶部