摘要
通过单剂的LC-P线计算混剂的期望LC-P线和相加作用区间(期望死亡率±20%期望死亡率),并与混剂的实测LC-P线和95%置信区间一起作图;分解混剂的LC50值为相应单剂的浓度,并由浓度对应的死亡率计算共毒因子;用单剂LC50值95%置信区间的上限/下限与混剂LC50值95%置信区间的下限/上限计算共毒系数区间等方法来分析共毒系数在杀虫剂混用评判中的偏差及校正方法。结果发现①当整个共毒系数区间在100以上或100以下时,共毒因子则大于+20或小于-20,实测LC-P线在相加作用区间外侧或期望LC-P线在95%置信区间外侧,表明期望LC-P线和实测LC-P线之间有毒力差异;②当整个共毒系数区间在100左右、跨越拮抗-相加-增效作用之间时,凡共毒因子大于+20或小于-20的,实测LC-P线在相加作用区间外侧或期望LC-P线在95%置信区间外侧,同样表明期望LC-P线和实测LC-P线之间有毒力差异;③凡共毒因子在-20与+20之间的,虽然用LC50值计算的共毒系数明显大于100,但相加作用区间和95%置信区间重叠,期望LC-P线和实测LC-P线彼此交缠,表明2条LC-P线之间没有毒力差异。因此,只有当共毒系数大于100,同时共毒因子大于20时,才能确认混剂对供试害虫的增效作用。
In order to analyse the deviation of co-toxicity coefficient among evaluation of joint action of pesticides against pests and the method of its correction,expected LC-P line of the mixtures and the region of additive action（expected mortality ±its 20%） were calculated according to the tested LC-P line of each insecticide,and then expected LC-P line with the region of additive action and tested LC-P line with the 95% confidence interval were drawn.Co-toxicity factors were calculated by the mortality coming from the corresponding concentration of each insecticide in mixture and from LC50 decomposition of the mixture.Regions of the co-toxicity coefficients were calculated by upper/lower limit of the 95% confidence interval of each individual insecticide and upper/lower limit of the mixture.The results were follows： ①The toxicity of mixture was different between the expected LC-P line and tested LC-P line when the region of co-toxicity coefficient was over 100 or below 100,the co-toxicity factor was more than ＋20 or less than-20,and the tested LC-P line was out of the region of additive action or the expected LC-P line was outside the 95% confidence interval;②The toxicity was also different between the two lines when the region of co-toxicity coefficients was about 100,the co-toxicity factor was more than ＋20 or less than-20,and the tested LC-P line was out of the region of additive action or the expected LC-P line was outside the 95% confidence interval;③The toxicity was not significantly different between the two lines when the region of co-toxicity coefficients was obviously over 100 and the co-toxicity factor was between ＋20 and-20.（There was overlap between the region of additive action and the 95% confidence interval,and the expected LC-P line and tested LC-P line crossed each other）.Therefore,in order to obtain a synergistic mixture of pesticide,the co-toxicity coefficient must be larger than 100 and the co-toxicity factor be larger than 20.
出处
《江苏农业学报》
CSCD
北大核心
2010年第6期1238-1246,共9页
Jiangsu Journal of Agricultural Sciences
基金
江苏省农业科技自主创新基金项目[CX(09)624]
关键词
杀虫剂混配
共毒系数
增效作用
偏差
校正
pesticide mixture
co-toxicity coefficient
synergism
deviation
correction
