摘要
目的为绿原酸(CGA)用于乳腺癌的治疗提供依据。方法将不同浓度的CGA干预乳腺癌MCF-7细胞;采用四甲基偶氮唑蓝(MTT)法检测CGA对MCF-7细胞的生长抑制率,流式细胞术检测MCF-7细胞凋亡率、细胞周期及细胞周期素(Cyclin)D1表达。结果 0.25、0.5 mg/ml CGA作用MCF-7细胞48 h后,使细胞阻滞于G1/G0期,两组的G1/G0期细胞较对照组明显增加(P<0.01);0、0.25、0.5 mg/ml CGA处理MCF-7细胞48 h后,Cyc-lin D1的平均荧光强度比(MFIR)分别为9.64±0.18、9.15±0.22、8.10±0.28(P=0.001)。结论 CGA可抑制MCF-7细胞增殖,使细胞阻滞于G/G期;其机制可能与下调Cyclin D1表达有关。
Objective To provide basis for the treatment of breast cancer by ehlorogenie acid （CGA）. Methods The breast cancer cell MCF-7 were treated with different concentrations of CGA ; the effects of CGA on the growth of MCF- 7 cells were studied by MTr assay ,the cell apoptosis, cell cycle and the expression of Cyclin Dlwere analyzed by flow ey- tometry. Results After treated with 0.25,0.5 mg/ml CGA for 48 hs, the cell cycle was arrested at G1/G0phase, the pro- portion of MCF-7 ceils at G1 phase of CGA group increased significantly compared to the control group（ P 〈 0.01 ） ; the mean fluorescence intensity ratio （MFIR） of Cyelin D1 was 9.64 ±0.18,9.15 ± 0.22,8.10± 0.28 （P = 0. 001 ） after the treatment of 0,0.25,0.5 mg/ml CGA. Conclusions CGA can inhibit the proliferation of MCF-7 ceils and induce Gl phase arrest in the cells;the mechanism possibly was down-regulating the expression of Cyelin D1.
出处
《山东医药》
CAS
北大核心
2010年第47期12-14,共3页
Shandong Medical Journal
基金
云南省卫生科技项目(2009NS035)
云南省科技厅联合基金项目(2007C0024R)
