摘要
目的:采用体外炎症模型研究瓜子金有效部位群抗炎作用机制。方法:建立脂多糖(LPS)诱导巨噬细胞系RAW264.7细胞体外炎症模型,研究不同浓度瓜子金有效部位群对一氧化氮(NO)、肿瘤坏死因子-α(TNF-α)、白细胞介素-6(IL-6)的释放及对TNF-α和IL-6 mRNA表达的影响。结果:瓜子金有效部位群能显著降低LPS诱导的RAW264.7细胞NO,IL-6及TNF-α的释放,显著降低TNF-α及IL-6 mRNA的表达。结论:瓜子金有效部位群抗炎作用与其减少巨噬细胞炎症介质的生成与释放及降低TNF-α和IL-6 mRNA的表达密切相关。
Objective： The inflammatory model in vitro was used to evaluate the anti-inflammatory mechanism of Polygala japonica effective ingredients.Method： RAW264.7 macrophage line was induced by lipopolysaccharide（LPS） to set up the inflammatory model.To study the effect of P.japonica effective ingredients on the inflammation factors by assaying the concentration of nitric oxide（NO）,tumor necrosis factor-α（TNF-α） and interleukin-6（IL-6）.The mRNA expression of TNF-α and IL-6 was assayed with real-time quantitative polymerase chain reaction（PCR） too.Result：P.japonica effective ingredients could significantly reduce the release of NO,IL-6 and TNF-α in LPS-induced RAW264.7 macrophage line,and obviously decrease the mRNA expression of TNF-α and IL-6,too.Conclusion：The anti-inflammatory mechanism of P.japonica effective ingredients may be related to reducing the release of inflammation factors in macrophage cell and inhibiting the mRNA expression of TNF-α and IL-6.
出处
《中国实验方剂学杂志》
CAS
北大核心
2011年第2期131-134,共4页
Chinese Journal of Experimental Traditional Medical Formulae
