摘要
目的:考察3种定痫胶囊加服维生素组合(受试药物Ⅰ,Ⅲ,Ⅴ)与含维生素组合的定痫胶囊(受试药物Ⅱ、Ⅳ、Ⅵ)的抗癫痫作用并对药效进行比较研究。方法:昆明种小鼠120只,雌雄各半,随机分为12组。癫痫大发作4组:戊四氮组、苯巴比妥钠组、受试药物Ⅰ组、受试药物Ⅱ组;癫痫小发作4组:戊四氮组、丙戊酸钠组、受试药物Ⅲ组、受试药物Ⅳ组;精神运动性癫痫发作4组:匹鲁卡品组、苯巴比妥钠组、受试药物Ⅴ组、受试药物Ⅵ组,每组10只,分别经戊四氮ip和sc、匹鲁卡品ip制备癫痫大发作、小发作及精神运动性发作模型,观察并记录给药后30 min内小鼠的惊厥潜伏期、惊厥次数及死亡时间。结果:Ⅰ与Ⅱ均显著延长癫痫大发作小鼠的死亡时间(P<0.01);Ⅱ能显著延长癫痫大发作小鼠惊厥潜伏期(P<0.01)。Ⅲ与Ⅳ均显著减少癫痫小发作小鼠的惊厥次数(P<0.01);显著延长癫痫小发作小鼠的死亡时间(P<0.01),Ⅳ降低小鼠死亡率(P<0.01)。Ⅴ与Ⅵ均显著长精神运动性癫痫小鼠的死亡时间(P<0.05)。结论:6种受试药物分别能显著治疗由戊四氮、匹鲁卡品诱发的癫痫大发作、小发作及精神运动性发作;在治疗癫痫小发作时,含维生素组合的菖连定痫胶囊作用优于菖连定痫胶囊+维生素组合。
Objective：To Compare the antiepileptic effect of vitamin plus Dingxian capsule with Dingxian capsule containing vitamin.Method：One hundred and twenty Kunming mice were randomly divided into 12 groups（PTZ group,sodium phenobarbital group,tested drugs Ⅰ group Ⅱ group;PTZ group,Valproate group,tested drugs Ⅲgroup,Ⅳgroup;pilocarpine group,sodium phenobarbital group,tested drugs Ⅴgroup Ⅵgroup）,n = 10 each respectively.With pentylenetetrazol and pilocarpine,epilepsy grand mal preparation,small seizures and psychomotor seizures model,were established.The seizure time within 30 minutes,frequency and time of death convulsions were observed and recorded.Result： Ⅰ and Ⅱ significantly prolonged the time of death（P0.01）;Ⅱ couldsignificantly prolong epileptic seizures latency in mice（P0.01）.Ⅲ and Ⅳ could significantly reduce seizure frequency（P0.01）,prolong the time of death（P0.01）,Ⅳ lowered seizures rate in mice（P0.01）.Ⅴand Ⅵ Could extend significantly the time of death for psychomotor epilepsy（P0.05）.Conclusion：Six tested drugs have significant effect for treatment of grand mal epilepsy,petit mal and psychomotor seizures induced by PTZ and pilocarpine.Dingxian capsule containing vitamin has a better role than vitamin plus Dingxian capsules for treament in seizures mice.
出处
《中国实验方剂学杂志》
CAS
北大核心
2011年第2期156-159,共4页
Chinese Journal of Experimental Traditional Medical Formulae
基金
河北省科技攻关项目(05276101D-88)
河北教育厅科学研究计划项目(y2009315)
河北省卫生厅医学科学研究重点课题计划项目(0501520090570)
河北省中医药管理局科研计划项目(2008072)
