摘要
利用吸附法将柱状假丝酵母菌脂肪酶(Candida rugosa lipase,CRL)固定于SBA-15介孔分子筛上,在搅拌槽反应体系中催化拆分外消旋萘普生甲酯的水解反应,获得了光学纯对映体(S)-萘普生,考察了SBA-15性能和酶固定量对初始反应速率、产量、转化率、对映体过剩(eep)和对映体选择性(E)的影响。结果表明,介孔分子筛SBA-15性能对固定化酶催化反应速率有显著影响;反应产量和转化率随酶固定量的增加而线性增大;固定化酶催化反应220 h的转化率达到45.8%,eep达到100%,E200,初始反应速率和对映体选择性都大大高于自由酶的催化性能。
Candida rugosa lipase（CRL） was immobilized on molecular sieve mesoporous silica SBA-15 by adsorption method for applying to lipase-catalyzed chiral resolution of racemic naproxen methyl esters to produceoptically pure（S）-naproxen.The effect of SBA-15 characteristics and immobilized amount of CRL on the kineticresolution were investigated in terms of initial reaction rate,production,conversion,enantiometric excess andenantioselectivity.It was found that catalytic activity of immobilized CRL was effected by SBA-15 characteristics.（S）-naproxen production and conversion increased linearly with the amount of immobilized CRL.The conversionreached to 45.8% after 220 hours of reaction and 100% of enantiometric excess（eep） and 200 ofenantioselectivity（E） were obtained.In addition,the initial reaction rate was 30 times higher than that of free CRL.Enantioselectivity of free CRL was only as much as 80%.This suggests that high production of pure（S）-naproxencan be achieved by catalytic resolution of racemic naproxen esters with CRL immobilized on SBA-15.
出处
《化学通报》
CAS
CSCD
北大核心
2011年第1期61-66,共6页
Chemistry
基金
国家自然科学基金项目(20406014)
国家重点基础研究计划(973项目)(2009CB2199006)资助
