摘要
利用同源克隆和RACE方法在商陆(Phytolacca acinosa)中得到一个推测的小G蛋白ArfGTPase激活蛋白ArfGAP(ArfGTPase activating protein)的全长cDNA序列PaAGAP.PaAGAP序列编码332个氨基酸.蛋白含有保守的锌指结构域(CX2CX16 CX2C类)和C2结构域.实时荧光定量PCR表明在寒、旱、盐和重金属的不同时间胁迫下,PaAGAP可以被诱导,表达量有不同程度的上调.由此推测PaAGAP可能是通过快速启动转录和翻译,使ARF失活,抑制植物生长素极性运输,来参与植物逆境反应的.
A full-length cDNA of an ArfGAP（PaAGAP） encoding 332 amino acids was cloned from Phytolacca acinosa by using methods of homology-based cloning and RACE.PaAGAP is predicted to contain a CX2CX16 CX2C type zinc finger domain and a C2 domain.Real-time PCR analysis revealed that the expression of PaAGAP was up-regulated in response to low temperature,osmotic,salt,and heavy metal stresses,indicating a potential role of PaAGAP in the plant stress response that involves the inactivation of ARF and the inhibition of polar auxin transport.
出处
《中国科学院研究生院学报》
CAS
CSCD
北大核心
2011年第1期116-124,共9页
Journal of the Graduate School of the Chinese Academy of Sciences
基金
国家高技术研究发展计划(863)项目(2006AA10Z407
2007AA021404)
中国科学院研究生院院长基金B类项目(085102FN00)资助
关键词
商陆
ARFGAP
锌指
逆境
表达
Phytolacca acinosa
ArfGAP
zinc finger
stress
expression
