摘要
目的探讨大蒜素对体外培养的人宫颈腺癌Hela、鳞癌Cask i细胞半胱氨酸蛋白酶(Caspase)-3、8、9的表达和活性变化对宫颈癌细胞生长的影响及其作用机制。方法2008年1月至2009年1月在河北医科大学附属第二医院通过体外培养的人宫颈腺癌Hela、鳞癌Cask i细胞,MTT法检测大蒜素作用后细胞的生长抑制率,分光光度法和RT-PCR方法分别检测大蒜素对细胞Caspase-3、8、9活性和表达的影响。结果用6.25、12.5、25、50、75、100mg/L的大蒜素处理Hela、Cask i细胞24、48、72h后,随着药物浓度和作用时间的增加,抑制率增高。分光光度法检测大蒜素作用48h后,Hela细胞Caspase-3、8、9活性的OD实验组/OD对照组分别是对照组的2.32、2.26、1.53倍;Cask i细胞是对照组的2.28、1.69、2.08倍(P<0.05)。RT-PCR法检测大蒜素作用12h后,Caspase-3、8、9mRNA基因扩增产物与β-actin基因扩增产物电泳带的吸光度比值明显升高,Hela细胞为对照组的1.92、1.47、1.57倍,Cask i细胞为对照组的1.87、1.55、1.66倍(P<0.05)。结论大蒜素对宫颈癌Hela和Cask i细胞有抑制作用,随时间和剂量增加抑制率上升。
Objective Our research compares between before and after allicin treatments on human cervical cancer cell lines Hela and Caski in vitro and expressions of Caspase-3,8,9, activity of Caspase-3,8,9 enzyme, and then discusses the effect of allicin on human cervical cancer cell lines and its mechanism,which can provides a pre-clinical evidence of adjuvant therapy to maglignant tumors of allicin in clinic. Methods Between January 2008 and January 2009 in the Second Affiliated Hospital of Hebei Medical University human cervical cancer cell lines Hela and Caski in vitro were treated with allicin. Growth inhibition rates of cells were determined with MTT assay. Caspase-3 ,8 ,9 activity changes before and after allicin treatment were determined by spectrophotometer. Expressions of Caspase-3,8,9 before and after alliein treatment by Osemi-quantity RT-PCR. Results Inhibitory rates of Hela and Caski cells after 24,48,72h allicin （6. 25,12. 5, 25,50,75,100mg/L） treatments had a time-and-dose-dependent effect （ P 〈 0.05 ）. Caspase-3,8,9 activities were upregulated obviously after 48h allicin treatment. The ODallicin/ODco,troI of Caspase-3,8,9 of Hela was 2. 32,2. 26,1.53times than control respectively. The ODallleia/ODcontrol of Caspase-3,8,9 of Caski was 2. 28,1.69,2. 08 times than control respectively （ P 〈 0. 05 ）. Absorbance ratios of electrophoresis belts of gene amplification products of Caspase-3,8,9 and β- actin were ascended markedly from RT-PCR. Comparing with control group, they were 1.43,1.47 times for Hela and 1.39,1.55 times for Caski （ P 〈 0.05 ）. Conclusion Allicin could inhibit cell proliferation and enhance apoptosis in cervical cancers cell lines Hela and Caski in vitro. Inhibitory rates were in a dose-and-time-dependent manner.
出处
《中国实用妇科与产科杂志》
CAS
CSCD
北大核心
2011年第1期39-41,共3页
Chinese Journal of Practical Gynecology and Obstetrics
基金
河北省科技厅科技攻关课题(Z200910111)
