摘要
将不同质量浓度的LaC l3加入楸树培养基中,研究LaC l3对楸树组培苗生长的影响。试验结果表明,稀土化合物LaC l3对楸树组培苗生长有显著效应,继代培养适量质量浓度可促进茎段愈伤组织膨大,促进增殖芽的生长,提高增殖系数,但对增殖分化芽的诱导有明显的抑制作用,整体趋势显示出低质量浓度促进,高质量浓度抑制,效果以20 mg.L-1左右为宜;生根培养适量质量浓度能促进单株发根数增多,促进根的伸长生长,提高生根率与移栽成活,低质量浓度抑制根系的发生、降低移栽成活率,高质量浓度提高生根率与移栽成活率,以80 mg.L-1效应显著;LaC l3能提高生根苗叶片叶绿素质量分数,且呈正相关,并可加速组培苗的光合作用,促进组培苗的生长;适量质量浓度的LaC l3能提高生根苗POD活性,增强组培苗抗逆性,以40 mg.L-1活性最高。
An experiment was conducted to study the effect of lanthanum chloride （ LaCl3 ） on the in vitro plantlets of Catalpa bungei by adding different concentrations of LaCl3 to the subculture and rooting media. Results show that rare earth compound LaCl3 has an obvious effect on the in vitro plantlets of C. bungei. Appropriate concentration of LaCl3 in subculture medium can accelerate the growth of tissue on stem segments, and it is helpful for the increase of buds, leaves and multiplieation coefficient, but it can greatly restrain the differentiation of sprouts. The whole tendency shows a promotive effect at low eoneentrations and a restraining effect at high concentrations, with an optimal effect at 20 mg · L^-1. Appropriate concentration of LaCl3 in roofing medium can accelerate the elongation of roots and is helpful for the increase of number of roots, rooting rate and transplant survival rate. LaCl3 can restrain root induction and decrease transplant survival rate at low concentrations, but accelerate rooting rate and transplant survival rate at high concentrations, with an obvious effect at 80 mg · L^-1. LaCl3 can increase chlorophyll content of leaves and has a positive correlation with chlorophyll content, and accelerate the photosynthesis and growth of the plantlets. Appropriate amount of LaCl3 can improve the POD activity and resistibility of the plantlets, with a highest effect at 40 mg · L^-1.
出处
《东北林业大学学报》
CAS
CSCD
北大核心
2011年第1期31-33,共3页
Journal of Northeast Forestry University
基金
"十一五"国家科技支撑课题(2006BAD24B08
2006BAD01A1602)
农业科技成果转化项目(2008GB24320414)
国家林业局林木培育实验室基金项目(2005-03)
关键词
楸树
稀土
试管苗
生长
叶绿素
POD活性
Catalpa bungei
Rare earth compounds
Plantlets
Growth
Chlorophyll
POD activity
