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孕早期产前筛查孕妇血清ADAM12-S中位数倍数校正方法的建立 被引量：2

Establishment of adjusted value for multiple of median of ADAM12-S concentration in prenatal serum screening during early pregnancy
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摘要 目的建立孕早期产前筛查孕妇血清解整合素金属蛋白酶12分泌型(a disintegrin and metalloprotease,ADAM12-S)中位数倍数的校正方法。方法随机选择2008年在本中心自愿参加孕早期唐氏综合征(DS)产前筛查并经随访证实分娩健康儿的孕妇孕8～13周血清样本577份,用时间分辨荧光免疫分析法检测血清ADAM12-S浓度。用SPSS 15.0软件构建ADAM12-S与孕龄间的回归关系,选最优模型计算中位浓度,得到ADAM12-S中位数倍数(multiple of the median,MoM)值。探讨MoM值与体重的关系,选取最优模型求出经体重调整后的MoM值。将ADAM12-S初始浓度与消除孕周和体重影响校正后的最终MoM值进行曲线拟合,选取最优模型最终得到ADAM12-S初始浓度与校正后MoM值的关系方程。结果 ADAM12-S中位数(Y)与孕周(t)呈正相关,经s曲线模型拟合后的中位数方程为:Y=e8.412-24.534/t;ADAM12-S MoM值中位数(Y)与体重中位数(z)存在负相关,经指数模型拟合后的方程为:Y=1.603*e-0.009*z;ADAM12-S初始浓度(X)与孕周和体重校正后的最终MoM值(Y)的关系方程为Y=0.036*X0.552。结论 ADAM12-S初始浓度与最终MoM值的关系方程的建立,对临床开展ADAM12-S筛查DS妊娠的研究有重要意义。 Objective To establish the adjusted value for multiple of the median（MOM） of a disintegrin and metalloprotease 12-secreting-type（ADAM12-S） in prenatal serum screening for Down′s syndrome.Methods The parturients,who were voluntary participants of prenatal screening program for Down′s syndrome during 2008 and were confirmed to labor healthy newborn by follow-up visit,were randomly enrolled in this study.Totally 577 serum samples of the first trimester（from 8 to 13 weeks） were collected and the concentration of ADAM12-S was determined by time-resolved fluorimmunoassay（TRFIA）.The regressive relationship between concentration of ADAM12-S and gestational age was analyzed with SPSS 15.0 software and then the optimal model was selected for the calculation of MOM value of ADAM12-S.The MOM value was adjusted on the basis of the body weight of parturients.The curve fitting of ADAM12-S concentration and the adjusted MOM value were conducted and the model was optimized for the equation by eliminating the influence of gestational age and body weight.Results A positive correlation between the MOM value of ADAM12-S（Y） and gestational age（t） was shown,and the equation was Y=e8.412-24.534/t,while a negative correlation between MOM value of ADAM12-S（Y） and body weight of parturients（z） was found,and the equation was Y=1.603＊e-0.009＊z.The equation of concentration of ADAM12-S（Y） and the adjusted MOM value（X） was Y=0.036＊X0.552.Conclusions The equation between the initial concentration of ADAM12-S and the adjusted MOM value was established.It should be considerably important for the screening program of Down′s syndrome in primary clinical hospital for newborn.
作者 蒋涛 吕伶 张晓娟 孙云 徐倩君 许争峰
机构地区 南京医科大学附属南京妇幼保健院产前诊断中心
出处 《临床检验杂志》 CAS CSCD 北大核心 2011年第1期50-52,共3页 Chinese Journal of Clinical Laboratory Science
基金 南京市医学科技发展项目(ZKX08001 ZKX06011) 南京市科技局项目(200801094)
关键词 解整合素金属蛋白酶12分泌型 唐氏综合征 产前筛查 a disintegrin and metalloprotease 12-secreting-type Down′s syndrome prenatal screening
分类号 R596.1 [医药卫生—内科学] R446.1 [医药卫生—诊断学]
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参考文献9

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二级参考文献8

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共引文献14

同被引文献19

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  • 3许飞,许婷姝,郭彩琴,等.解整合素金属蛋白酶12在唐氏综合征产前筛查中的初步研究[J].中国优生优育,2009,15(4):208.
  • 4Laigaard J, Srensen T, Frohlich C, et al. ADAM12: a novel first - trimester mater -nal serum maker for Down symdrome [J]. Prenat Diagn, 2003, 23 (13) : 1086 -1091.
  • 5Laigaard J, Christiansen M, Frohlich C, et al. The level of ADAM12- S in maternal serum is an early first -trimester maker of fetal trisomy 18[J]. Prenat Diagn, 2005, 25 (1): 45-46.
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  • 8Sabine G, Alexander M, Frederik M, et al. Preeclampsia: increased expression of souble ADAM 12 [J]. J Mol Med, 2005, (83) : 887.
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  • 10唐婕,周昌菊,谷永红,马薇.解整合素-金属蛋白酶12在子痫前期患者胎盘组织中的表达及意义[J].国际病理科学与临床杂志,2007,27(6):481-484. 被引量：4

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临床检验杂志
2011年 第1期

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