摘要
目的建立呈胰岛素抵抗的HepG2细胞模型。方法将HepG2细胞置于10-7mol／L胰岛素培养液中24小时，使HepG2细胞胰岛素受体充分下调，检测并比较下调和未下调的HepG2细胞、糖、脂类代谢水平，发现：下调HepG2细胞（DR-HepG2）胰岛素受体减少了56％，将DR-HepG2细胞置于不同浓度胰岛素培养液中，胰岛素受体数仍明显减少，当培养液中胰岛素的浓度为10-7mol／L时，DR-HepG2细胞胰岛素受体数减少到17％，其14C-葡萄糖、14C-醋酸盐掺合量明显低于未下调的HepG2细胞（NDR-HepG2）。将DR-HepG2细胞置于不含胰岛素DMEM培养液中48小时，再用不同浓度的胰岛素刺激24小时。结果DR-HepG2细胞14C-葡萄糖、14C-醋酸盐掺合量仍明显低于NDR-HepG2细胞。结论将HepG2细胞置于10-7mol／L胰岛素环境中24小时，该细胞对胰岛素的作用产生抵抗，该胰岛素抵抗状态可维持48小时。
Objective To establish insulin-resistant HepG2 cell line. Methods HepG2 cells were exposed to 10-7mol/L insulin for 24 hours, washed with DMEM (pH4. 0) and finally with pre-cold PBS. Results it has been demonstrated that a state of insulin resistance has been induced in HepG2 cells with the evidences that the residual 125Iinsulin binding was reduced by 56% and the amounts of incorporation of 14C-glucose, 14C-acetate in down-r gulated HepG2 cells (DR-HepG2) were lower than in control cells (NDR-HepG2 cell). Though DR-HepG2 cells were free from stimulation for 48 hour by insulin, the amounts of incorporation of the above mentioned labelled substances in DRHepG2 cells were remaining lower than those in NDR-HepG2 cells. Conclusion HepG2 cells exposed to 10-7mol/Linsulin for 24 hours were able to induce a state of insulin resistance which could be maintained for 48 hours.
出处
《中国糖尿病杂志》
CAS
CSCD
1999年第4期198-200,共3页
Chinese Journal of Diabetes
基金
本课题得到卫生部
上海市科委启明星计划的资助
