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巨噬细胞内结核分枝杆菌DNA的检测及实验评价 被引量:2

Detection and test evaluation of Mycobacterium tuberculosis DNA in macrophages by polymerase chain reaction
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摘要 The Mycobacterium tuberculosis NDA in the macrophages from the different clinical specimens,such as ascites,pleurorrhea,CSF,BALF and blood,is detected with polymerase chain reaction (PCR).The results indicate that the test specificity is 96%,the precision is 92% and the sensitivity is 88%.The Separated and purified macrophage in a good specimen in the detecting TB-DNA by PCR.The test specificity and precision are high.The test is a good method of the diagnosis of the tuberculosis,specially the patient with the outer-pulmonary tuberculosis.The exact significance in the basic theory and the clinical practice are not affirmed.so it will be investigated further. The Mycobacterium tuberculosis NDA in the macrophages from the different clinical specimens,such as ascites,pleurorrhea,CSF,BALF and blood,is detected with polymerase chain reaction (PCR).The results indicate that the test specificity is 96%,the precision is 92% and the sensitivity is 88%.The Separated and purified macrophage in a good specimen in the detecting TB-DNA by PCR.The test specificity and precision are high.The test is a good method of the diagnosis of the tuberculosis,specially the patient with the outer-pulmonary tuberculosis.The exact significance in the basic theory and the clinical practice are not affirmed.so it will be investigated further.
出处 《中国卫生检验杂志》 CAS 1999年第5期334-336,共3页 Chinese Journal of Health Laboratory Technology
基金 贵州省卫生厅科学基金
关键词 巨噬细胞 结核分枝杆菌 DNA 检测 Macrophage TB-DNA PCR Diagnosis.
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