摘要
目的观察Smad4反义寡核苷酸(antioligodeoxynucleotide,antiODN)对体外高糖培养的人肾小球系膜细胞(human glomerular mesangial cells,HGMC)分泌纤连蛋白(fibronectin,FN),层黏连蛋白(laminin,LN)的影响。方法用脂质体法将正义、反义、错义Smad4寡核苷酸瞬时转染体外培养的HGMC,并进行高糖培养,同时设立正常糖组、高糖组、甘露醇组、脂质体组为对照组,用ELISA法检测培养上清液中FN、LN的含量,进行统计学分析。结果转染Smad4反义寡核苷酸的HGMC分泌的FN、LN量与正常对照组相比无统计学意义(P>0.05),而与高糖组、正义组、错义组相比差异有统计学意义(P<0.01)。结论随着高糖作用的时间延长,体外培养的HGMC分泌的FN、LN量逐渐增加,而转染Smad4 antiODN可以抑制高糖的刺激,减少FN、LN的表达,从而减少系膜细胞细胞外基质的堆积,为延缓肾脏纤维化提供了新的途径。
[Objective] To observe the effects of Smad4 antioligodeoxynucleotide （antiODN） on the excretion of fibroneefin （FN） and lanfinin （LN） by human glomerular mesangial ceils （HGMC） under hyperglycemia. [Methods] Anfisense, sense and misssense Smad40DNs were transferred transiently into human glomerular mesangial cells with liposome. HGMCs were cultured with high glucose and normal glucose, high glucose, mannitol and lipofectamine 2000 were used as control. The secretion of fibronecfin and laminin by HGMCs in supernatants of culture media was evaluated by ELISA. [Results] Compared with high glucose, antisense and missense groups, concentrations of FN and LN decreased significantly in antisense group （P 〈0.05）, the value nearly approach the normal （.P 〉 0.05）. [ Conclusion] FN and LN secreted by HGMC cultured under high-glucose conditions were increased in a time-dependent manner. Antisense Smad40DN can reduce the FN and LN secretion in HGMC cultured with high glucose.The study supply a new method of diabetic nephropathy.
出处
《中国现代医学杂志》
CAS
CSCD
北大核心
2011年第1期10-13,18,共5页
China Journal of Modern Medicine
基金
辽宁省自然基金(No:20032068)
辽宁省教育厅科学技术基金(No:202013140)
