摘要
利用RAPD分子标记技术对我国18 个苜蓿地方品种和北美9 个苜蓿基本种质来源的代表品种进行了分析,根据分析结果计算了品种间的欧氏遗传距离,并进行聚类分析。结果表明:我国18 个苜蓿地方品种间的平均遗传距离为1.56,以1.5 的遗传距离进行划分可将我国18 个苜蓿地方品种分为7 组,品种间的亲缘关系与地理分布有一定的相关性。北美9 个苜蓿品种间的平均遗传距离为1.68,聚类分析结果再次证明,M. sativa ssp. sativa 和M. sati-va ssp. varia 两个亚种之间的遗传差别相对较小,而M. sativa ssp. falcata 亚种与前两个亚种之间的遗传差异相对较大。与北美9 个苜蓿品种相比,我国苜蓿地方品种间的遗传差异较小,原因是我国苜蓿地方品种的地理分布相对较窄,品种间基因交流的机会较多,品种的遗传组成趋同。RAPD标记聚类结果与种子贮藏蛋白标记聚类结果具有一定的相似性,也存在一些不一致的情况,其原因是这两种标记反映了苜蓿基因组中不同基因位点的变异情况,而不同品种间在这些不同的基因位点可能存在不同的差异。
Genetic distance and relationships of the 18 alfalfa local varieties in China and the 9 alfalfa varieties representing the 9 basic alfalfa germplasm sources in Northern American were analyzed using RAPD markers. Results show that average distance of 18 alfalfa local varieties in China is 1.56, and the 18 varieties can be clustered into 7 groups when the genetic distances of 1.5 are used as critical value. Results of cluster analysis indicate that relationships between the 18 alfalfa varieties in China are related to their geographic distribution. Average distance of the 9 varieties in Northern American is 1.68, and results of cluster analysis verify that Medicago sativa ssp. varia has more similarity with Medicago sativa ssp. sativa than Medicago sativa ssp. falcata. Compared with the 9 varieties in Northern American, difference among the 18 varieties in China are smaller, and this result is related to the narrow geographic distributions and frequent gene communications of the 18 alfalfa local varieties in China. Relationships between the 18 alfalfa local varieties in China revealed by RAPD markers and seed storage protein markers are similar, and there are also some exceptions. Reason for these exceptions is that loci revealed by RAPD and seed storage protein markers are different, and difference between varieties may be not same on different loci.
出处
《草业学报》
CSCD
1999年第3期46-53,共8页
Acta Prataculturae Sinica
