摘要
利用纯化的Ｂｔ毒素蛋白（６７ｋＤａ）作为标准蛋白和免疫抗原，建立了检测转基因抗虫棉植株内Ｂｔ毒蛋白含量的抗体夹心ＥＬＩＳＡ方法。检测极限１μｇ·Ｌ－ １，线性范围１－ １５μｇ·Ｌ－ １，板内误差Ｃ．Ｖ．＜ ５％ ，板间误差Ｃ．Ｖ．＜ ５％ ，平均样品回收率９４．９４％ 。经稀释度试验等质量控制试验，表明所建立的ＥＬＩＳＡ方法是可靠的，可用于转基因棉花植株中Ｂｔ毒素蛋白含量的测定。
An antibody sandwich enzyme linked immunosorbent assay (ELISA) was developed to detect and quantitate the Bacillus thuringiensis (Bt) insecticidal protein expressed in transgenic insect resistant cotton using a purified Bt toxin (67kDa) as standard protein and antigen. The minimal detectable concentration of purified Bt toxin was 1μg·L -1 and the linear assay range of the method was 1μg·L -1 to 15μg·L -1 .Both the errors of intra and interplate(s) were <5%,and the mean recovery rate was 94.94%.It was indicated by quality controal experiments that this ELISA method was stable and reliable ,and it can be used in the detectiomn of Bt toxin content expressed in transgenic cotto.
出处
《棉花学报》
CSCD
北大核心
1999年第5期259-267,共9页
Cotton Science
基金
国家计委"八五"攻关项目
