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急性肝衰竭大鼠血清诱导骨髓间充质干细胞表达甲胎蛋白和白蛋白 被引量:3

Expression of alpha-fetoprotein and albumin in bone marrow mesenchymal stem cells induced by hepatic failure rat serum
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摘要 背景:研究发现肝细胞生长因子、成纤维细胞生长因子4和表皮细胞生长因子等可诱导骨髓间充质干细胞向肝细胞分化,关于肝衰竭大鼠血清对骨髓间充质干细胞的诱导分化作用尚未见报道。目的:观察急性肝衰竭大鼠血清对骨髓间充质干细胞的诱导分化作用。方法:采用密度梯度离心法从SD大乳鼠股骨、胫骨分离培养骨髓间充质干细胞,体外扩增培养。采用急性肝衰竭大鼠血清诱导培养骨髓间充质干细胞,以常规培养液诱导为对照,观察细胞形态的变化,诱导培养14d采用免疫细胞化学方法检测甲胎蛋白、白蛋白的表达。结果与结论:急性肝衰竭大鼠血清诱导培养14d,可见骨髓间充质干细胞发生明显的形态学改变,镜下观察细胞变得稍大而扁平,呈类上皮样细胞,甲胎蛋白和白蛋白表达阳性率分别为(54.8±7.64)%和(45.9±9.68)%;对照组细胞未发生形态学改变,无甲胎蛋白和白蛋白的表达。证明了急性肝衰竭大鼠血清对骨髓间充质干细胞的诱导分化作用,可诱导骨髓间充质干细胞表达甲胎蛋白和白蛋白。 BACKGROUND:It was found that hepatocyte growth factor,fibroblast growth factor-4 and epidermal growth factor could induce differentiation of bone marrow mesenchymal stem cells(BMSCs) into hepatocytes.There were no reports on the inducing effect of hepatic failure rat serum on BMSCs.OBJECTIVE:To study the effects of hepatic failure rat serum on the induced differentiation of BMSCs.METHODS:BMSCs were isolated from SD rat femur and tibia by density gradient centrifugation and culture in vitro.BMSCs were cultured in DMEM containing the hepatic failure rat serum and conventional culture medium as control.The morphological changes of cells were observed and the expression of alpha-fetoprotein and albumin was measured by immunocytochemistry method on 14 days.RESULTS AND CONCLUSION:Obvious morphological changes were observed on 14 day,which showed that BMSCs became slightly large and flat cells as epithelial cells.The positive rates of alpha-fetoprotein and albumin were(54.8±7.64)% and(45.9±9.68)% respectively.Cells in the control group did not have morphological changes,no alpha-fetoprotein and albumin expression.The results showed that BMSCs can be induced to express alpha-fetoprotein and albumin by hepatic failure rat serum.
出处 《中国组织工程研究与临床康复》 CAS CSCD 北大核心 2010年第45期8369-8372,共4页 Journal of Clinical Rehabilitative Tissue Engineering Research
基金 长春市科技发展计划项目(08SF07) 脐血干细胞治疗终末期肝病的临床应用研究~~
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参考文献20

  • 1李焕萍,刘春蓉,张永亮.骨髓间充质干细胞在医学领域中的研究与应用[J].中国组织工程研究与临床康复,2007,11(28):5622-5625. 被引量:27
  • 2胡资兵,曾荣,郭伟韬,林颢.骨髓间充质干细胞诱导分化特征[J].中国组织工程研究与临床康复,2008,12(43):8561-8566. 被引量:20
  • 3Woodbury D,Reynclds K,Black IB.Adult bone marrow stromal stem cells:Express germline,ectodermal,endodermal,and mesodermal genes prior to neurogenesis.J Neurosci Res.2002; 69(6):908-917.
  • 4孙伟,胡亮,赵兴,李官成,付炳方,潘颂华.体外定向诱导小鼠骨髓间充质干细胞分化为神经元样细胞[J].安徽农业科学,2010,38(7):3492-3495. 被引量:6
  • 5Anderson PA,Muller-Borer BJ,Esch GL,et al.Calcium signals induce liver stem cells to acquire a cardiac phenotype.Cell Cycle.2007; 6(13):1565-1569.
  • 6Ohnishi S,Yarmgawa B,Tanaka K,et al.Transplantation of mesenchymal stem cells attenuates myocardial injury anddysfunction in a rat model of acute myocarditis.J Mol Cell Cardiol.2007;42(1):88-97.
  • 7Piccini JP,Hranitzky P.Diagnostic monitoring strategies in heart failure management.Am Heart J.2007;153(4 Suppl):12-17.
  • 8Iop L,Chiavegato A,Callegari A,et al.Different cardiovascular potential of adult-and fetal-type mesenchymal stem cells in a rat model of heart cryoinjury.Cell Transplant.2008;17(6):679-694.
  • 9Yamazaki S,Miki K,Hasegawa K,et al.Sera from liver faiure patients and a demethylating agent stimulate transdifferentiation of murine bone marrow cells into hepatocytes in coculture with nonparenchymal liver cells.J Hepatol.2003;39(1):17-23.
  • 10Schwartz RE,Reyes M,Koodie L,et al.Multipotent adult progenitor cells from bone marrow differentiate into functional hepatocyte-like cells.J Clin Invest.2002;109:1291-1302.

二级参考文献165

共引文献195

同被引文献19

  • 1陆海华,滕皋军,居胜红,孙军辉,李爱梅,张爱凤.小鼠骨髓间充质干细胞体内定向诱导分化与治疗肝功能损伤的实验研究[J].中华医学杂志,2007,87(4):223-227. 被引量:9
  • 2靳海英,任锋,丁美,赵军,张晶,陈煜,武志明,段钟平.N-乙酰半胱氨酸对猪急性肝衰竭血清肿瘤坏死因子-α、白介素-1β的影响[J].药品评价,2007,4(3):153-156. 被引量:5
  • 3Fang B,Shi M,Liao L,et al.Systemic infusion of FLK1+rmesenchymal stem cells ameliorate carbon tetrachloride-induced liver fibrosis in mice[J].Transplantation,2004,78(1):83-88.
  • 4Lee KD,Kuo TK,Whang-Peng J,et al.In vitro hepaticdifferentiation of human mesenchymal stem cells[J].Hepatology,2004,40(6):1275-1284.
  • 5Devine SM,Cobbs C,Jennings M,et al.Mesenchymalstem cells distribute to a wide range of tissues followingsystemic infusion into nonhuman primates[J].Blood,2003,101(8):2999-3001.
  • 6Jiang Y,Jahagirdar BN,Reinhardt RL,et al.Pluripotencyof mesenchymal stem cells derived from adult marrow[J].Nature,2002,418(6893):41-49.
  • 7Krause DS,Theise ND,Collector MI,et al.Multi-organ,multi-lineage engraftment by a single bone marrow-derivedstem cell[J].Cell,2001,105(3):369-377.
  • 8Vogel G.Stem cell research.Rat brains respond toembryonic stem cells[J].Science,2002,295(5553):254-255.
  • 9Theise ND,Badve S,Saxena R,et al.Derivation ofhepatocytes from bone marrow cells in mice after radiation-induced myeloablation[J].Hepatology,2000,31(1):235-240.
  • 10Schwartz RE,Reyes M,Koodie L,et al.Multipotent adultprogenitor cells from bone marrow differentiate into functionalhepatocyte-like cells[J].J Clin Invest,2002,109(10):1291-1302.

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