摘要
背景:灯盏花素能影响微血管舒张、减少缺血再灌注的损伤、降低血液黏滞性、对抗氧自由基损伤等,可能在皮瓣移植后治疗中起重要作用。目的:探讨灯盏花素对大鼠超长比例皮瓣成活的影响和可能的机制.方法:30只SD大鼠随机分为灯盏花素组、丹参组和对照组。在大鼠背部设计制备一蒂在尾端的8cm×2cm的超长全厚随意皮瓣,原位回植。灯盏花素组和丹参组在术后立即及之后每日分别腹腔内注射灯盏花素注射液4.5mL/(kg·d)及丹参注射液1.8mL/(kg·d),对照组给予1mL生理盐水。术后7d,观察皮瓣存活情况,对皮瓣远、中及近段进行组织学观察;取大鼠皮瓣中轴距蒂部3cm处组织,检测超氧化物歧化酶、丙二醛和一氧化氮水平。结果与结论:术后7d,灯盏花素组和丹参组皮瓣存活率高于对照组(P<0.05),其中灯盏花素组皮瓣存活率较高。灯盏花素组和丹参组近、中及远段组织水肿、坏死及炎症细胞浸润较对照组轻,其血管、成纤维细胞增生均明显优于对照组。灯盏花素组和丹参组超氧化物歧化酶及一氧化氮含量高于对照组(P<0.05),其中灯盏花素组最高;而灯盏花素组和丹参组丙二醛含量低于对照组,灯盏花素组最低。说明腹腔注射灯盏花素注射液对大鼠随意超长皮瓣成活有明显促进作用,作用机制可能与扩张血管、改善循环、清除自由基及降低脂质过氧化反应有关,其作用优于丹参注射液。
BACKGROUND：Breviscapine injection can affect microcirculation,decrease ischemia-reperfusion injury,decline blood viscosity,as well as anti oxygen free radical injury,which plays an important role in the treatment following flap transplantation.OBJECTIVE：To investigate the effects of breviscapine injection on the survival of random pattern skin flap with large length-to-width ratio in rats and the probable mechanism that contribute to this effects.METHODS：Thirty SD rats were randomly divided into the experimental groups and control group（n=10）.Random pattern skin flap of 8 cm × 2 cm（length：width = 4：1） in size was made on the back of each rat.Breviscapine injection（4.5 mg/kg）,danshen injection（1.8 mL/kg） and normal saline with the same dose were injected into the abdominal cavity of rats in the experimental groups and the control group immediately after the operation and the next seven days.The rats were killed at 7 days after operation.The survival rate of the flap,the tissue pathology from the proximal,middle and distal flaps,and the superoxide dismutase（SOD） activity,the level of malonyldialdehyde（MDA） and nitrogen monoxide（NO） were compared between these groups.RESULTS AND CONCLUSION：The mean survival rate of the flap in the experimental groups was higher than that in the control group at 7 days after operation,especially in the breviscapine injection group（P 0.05）.Histological observation showed that compared with the control group,the edema,necrosis and inflammatory cells infiltration were less,while the microvascular growth and the fiber hyperplasia were more obvious in the experimental groups,especially in the breviscapine injection group（P 0.05）.SOD activity in experimental groups were higher than that in the control group,while the level of MDA and NO were lower than that in the control group,especially in the breviscapine injection group（P 0.05）.The intraperitoneal use of breviscapine injection may promote the survival rate of the random pattern skin flaps with large length-to-width ratio in rats,and the possible mechanism for improvement may lies in that the breviscapine injection can expand the microvascular,improve circulation,reduce the level of oxygen free radical and lipid peroxidation.The effects of breviscapine injection to promote the survival rate in flaps were better than that of danshen injection.
出处
《中国组织工程研究与临床康复》
CAS
CSCD
北大核心
2010年第46期8620-8624,共5页
Journal of Clinical Rehabilitative Tissue Engineering Research
