摘要
目的研究氧化苦参碱对内毒素(LPS)作用下人牙周膜细胞(PDLC)白细胞介素-6(IL-6)和白细胞介素-1β(IL-1β) mRNA表达的影响,探讨氧化苦参碱抑制LPS所造成的牙周炎症反应的机制。方法体外分离及培养PDLC,实验组分别为LPS和不同质量浓度的氧化苦参碱溶液的不同组合,对照组为仅含1%FBS的DMEM培养液。采用逆转录聚合酶链反应(RT-PCR)检测IL-6和IL-1β mRNA的水平。结果质量浓度为25μg.mL-1 LPS能够有效地增强IL-6和IL-1β mRNA水平的表达,实验组高于对照组,差异有统计学意义,加入氧化苦参碱进行干预后实验组与对照组IL-6和IL-1βmRNA的表达无显著差异。结论氧化苦参碱可抑制LPS所造成的PDLC IL-6和IL-1βmRNA表达。
Objective To observe the effects of oxymatrine on the expression of interleukin-6（IL-6）,interleukin-1β（IL-1β） mRNA of human periodontal ligament cell（PDLC） stimulated by lipopolysaccharides（LPS）,and to discuss oxymatrine＇s inhibition mechanism on periodontal inflammation stimulated by LPS.Methods Firstly,isolate PDLC externally and culture them;for oxymatrine experimental group we used different combination of LPS and oxymatrine in different concentration,and for the matched group we use DEME nutrient solutions of 1% FBS.Then reverse transcription-polymerase chain reaction（RT-PCR） for checking the level of IL-6 and IL-1β mRNA.Results 25 μg·mL-1 LPS can significantly enhance the expression of both IL-6 and IL-1β mRNA＇s level,and oxymatrine could restrain above phenomena.Conclusion Oxymatrine can restrain the expression of IL-6 and IL-1β mRNA of human PDLC stimulated by LPS.
出处
《华西口腔医学杂志》
CAS
CSCD
北大核心
2010年第6期656-659,共4页
West China Journal of Stomatology
基金
福建省科技厅青年人才基金资助项目(2006F3051)
福建医科大学附属口腔医院重点学科建设学术发展基金资助项目[闽医大口腔(2008)39号]
关键词
氧化苦参碱
内毒素
牙周膜细胞
炎症
oxymatrine
lipopolysaccharides
periodontal ligament cell
inflammation
