摘要
壁式慢回转反应器(RWVS)是三维培养的常用装置,同时它也被广泛用于在地面模拟微重力效应对细胞的影响。目前,众多学者都在探讨RWVS为何能在一定程度上模拟部分微重力效应。不少相互独立的研究均表明,这种效应可能与细胞在RWVS中所受的力学微环境密切相关,包括低流场剪切力与微载体的运动等,其中微载体的运动又分为一级运动与次级运动两种。本文从微载体的受力分析出发,结合众多研究者的仿真和实验观测结果,对微载体的一级、次级运动及其研究方法做一个较为全面的梳理。
Rotating wall vessels（RWVS）,an ingenious apparatus for three-dimensional suspension culture,is widely used to build a simulated microgravity-effect on cell.Independent researchers have proposed hypotheses to illustrate why RWVS can simulate certain aspects of microgravity.Many of the hypotheses stated that the culture condition in RWVS is determined by the cellular mechanical environment which is a result of low fluid shear and microcarrier＇s motion.The microcarrier＇s motions consist of primary and secondary motions.In the light of the analysis of forces loaded by the microcarriers,some conclusions are drawn from the data on microcarriers＇ primary and secondary motions about which many simulations and observations have already been conducted.
出处
《生物医学工程学杂志》
EI
CAS
CSCD
北大核心
2010年第6期1433-1438,共6页
Journal of Biomedical Engineering
基金
国家自然科学基金资助项目(10672013)
关键词
模拟微重力效应
壁式慢回转反应器
细胞力学微环境
微载体
运动
Simulated microgravity-effect
Rotating wall vessels（RWVS）
Cellular mechanical environment
Mircocarrier
Motion
