摘要
目的:建立用高效液相色谱法测定六味安消胶囊中游离蒽醌、总蒽醌和结合蒽醌含量(以大黄素和大黄酚的总量计)的方法。方法:采用依利特C18柱,流动相为甲醇-0.1%磷酸溶液(80∶20),检测波长为254 nm。结果:大黄素、大黄酚分别在0.016 2～0.323 2μg和0.035 6～0.712 8μg范围内线性关系良好,游离蒽醌中大黄素和大黄酚的平均回收率分别为100.08%、99.11%,RSD分别为0.37%、2.70%;总蒽醌中大黄素和大黄酚的平均回收率分别为101.62%、100.54%,RSD分别为2.40%、1.37%。结论:HPLC法简便,准确,可作为六味安消胶囊的质量控制定量分析方法。
Objective： To develop an HPLC method for determination of free anthraquinone,total anthraquinone and binding anthraquinone in Liuwei Anxiao capsules.Methods： Hypersil C18 column（200 mm×5.0 mm,5 μm） was adopted.The mobile phase was methnol-0.1% phosphoric acid（80∶20）.The flow rate was 1 mL·min-1 and the detection wavelength was 254 nm.Results： The linear ranges of emodin and chrysophanol were 0.016 2～0.323 2μg and 0.0356～0.7128μg,respectively.The average recoveries were 100.08%（RSD=0.37%） for emodin,and 99.11%（RSD=2.70%） for chrysophanol in free anthraquinone,and 101.62%（RSD=2.40%） for emodin,and 100.54%（RSD=1.37%） for chrysophanol in total anthraquinone,respectively.Conclusion： The method is convenient,accurate and can be used for determination of the preparation.
出处
《贵阳医学院学报》
CAS
2010年第6期578-580,583,共4页
Journal of Guiyang Medical College
关键词
色谱法
高压液相
蒽醌类
大黄素
大黄酚
chromatrography
high pressure liquid
anthraquinones
emodin
chrysophanol
