摘要
目的：了解静脉输注脂肪乳剂是否会增加机体的脂质过氧化和改变机体血浆维生素Ｅ浓度，脂肪乳剂所致的脂质过氧化是否会造成机体组织细胞的ＤＮＡ损害。 方法：２２ 例健康男性志愿者进行两个阶段的随机交叉研究。禁食一晚后于清晨８：００ 抽血作为基础对照，然后分别连续静脉输注２０％ Ｉｎｔｒａｌｉｐｉｄ 或２０％ Ｖａｓｏｌｉｐｉｄ ４ｈ（甘油三酯输注速度：０．１５ ｇ／ｋｇ·ｈ－ １），两种脂肪乳剂输注之间相隔２周，并分别于输注２ ｈ、４ ｈ 时抽血测定血浆脂质过氧化终产物ｍ ａｌｏｎａｌｄｅｈｙｄｅ（ＭＤＡ）和４－ｈｙｄｒｏｘｙａｌｋｅｎａｌ的总量；测定血浆α－ｔｏｃｏｐｈｅｒｏｌ代表维生素Ｅ浓度；自实验前一天起连续收集３ 天尿，检测ＤＮＡ 损害终产物——８－Ｈｙｄｒｏｘｙｇｕａｎｉｎｅ（８－ＯＨＧｕａ）量，作为ＤＮＡ 损害程度的分析。 结果：静脉输注脂肪乳剂过程中，血浆ＭＤＡ和４－ｈｙｄｒｏｘｙａｌｋｅｎａｌ明显增高，输注后２４ ｈ 降至输注前水平，两种脂肪乳剂之间无统计学上差异，脂肪乳剂输注过程中血浆维生素Ｅ浓度无明显变化。两种脂肪乳剂应用前后，尿中８－ＯＨＧｕａ含量无变化。 结论：静脉输注脂肪乳剂可增加机体脂质过氧化。
Objectives:Lipid emulsions used in parenteral nutrition are prone to peroxidation that may be an important feature of oxygen associated tissue damage.In the present study,we determine whether lipid emulsions increase lipid peroxidation in vivo ,and evaluate endogenous oxidative damage to DNA in healthy subjects while receiving intravenous lipid emulsions. Methods:Twenty two healthy subjects were studied on two occasions at an interval of 2 weeks.Intralipid and Vasolipid were infused for 4 hours at a rate of 0.15 g TG/h,blood samples were taken before and during lipid infusion.We used a new colorimetric assay to measure the end products of lipid peroxidation,HPLC was performed to measure plasma concentration of α tocopherol;the urinary concentration of 8 Hydroxyguanine(8 OHGua) was measured by HPLC and isotope dilution mass spectrometry. Results:The plasma MDA and 4 hydroxyalkenal increased significantly after the infusion of lipid emulsions;the infusion lipid emulsions for 4 hours didnt result in a marked changes of plasma α tocopherol and 8 Hydroxyguanine levels. Conclusions:Lipid peroxidation increased after the infusion of lipid emulsions.The infusion lipid emulsions for 4 hours did not result in oxidative DNA damage and didnt influence the plasma α tocopherol levels.
出处
《肠外与肠内营养》
CAS
1999年第4期206-209,共4页
Parenteral & Enteral Nutrition
