摘要
内根-贝壳杉烯氧化酶(KO)是赤霉素合成代谢的关键酶。以苹果(Malus×domestica Borkh.)品种富士(Fuji)为供试材料,应用同源克隆和RACE方法从其茎尖中克隆到KO的cDNA全长序列,命名为MdKO,GenBank登录号:AY563549,其长度为1 859 bp。MdKO的开放性阅读框(ORF)编码514个氨基酸残基,推断其相对分子量为58.9 ku,等电点为7.63。氨基酸同源性分析表明,MdKO与已报道的其他植物的KO氨基酸序列具有较高的相似性;氨基酸聚类分析表明,苹果和梨首先聚类,其次是葡萄;序列结构分析表明MdKO属于细胞色素超家族P450系,具有细胞色素P450的血红素结构域FXXGXRXCXG和跨膜结构域;亚细胞定位分析发现MdKO可能位于内质网膜上。
Ent-Kaurene oxidase（KO） is a critical enzyme in the pathway of gibberellins biosynthesis.In this research,ent-kaurene oxidase gene in apple,designated as MdKO（GenBank accession number： AY563549）,was isolated from the apical tissue of Fuji apple stem.The full cDNA was 1 859 bp in length with an open reading frame（ORF）of 1 545 bp encoding a protein of 514 amino acids.The protein molecular weight and isoelectric point were predicted to be 58.9 ku and 7.63,respectively.Amino acids homology analysis indicated a high similarity with its counterparts in other species.Amino acids cluster analysis showed that KO from pear was clustered together with MdKO firstly,and followed by that from grape.Sequence analysis exhibited that MdKO belonged to cytochrome P450 superfamily and contained cysteine heme-iron ligand signature（FXXGXRXCXG）and transmembrane region.Moreover,MdKO was located in endoplasmic reticulum membrane.
出处
《果树学报》
CAS
CSCD
北大核心
2011年第1期1-7,共7页
Journal of Fruit Science
基金
山东省自然科学基金(Y2008D51)
青岛农业大学高层次人才科研基金
