摘要
目的评价福建省福州市生活饮用水及其水源水的体外遗传毒性。方法随机选择福州市5个区县(市)(分别为A区、B市、C区、D县、E市)各1家水厂,采集出厂水和水源水各200L,经XAD-2树脂柱收集其中的有机物,采用体外单细胞凝胶电泳试验和体外微核试验检测其遗传毒性。结果单细胞凝胶电泳试验测定O live尾矩发现B、D和E出厂水3个高剂量组[B:(1.321±0.50)、(1.310±0.82)、(1.209±0.74)μm;D:(1.297±0.48)、(1.289±0.76)、(1.235±0.69)μm;E:(1.553±0.65)、(1.534±0.68)、(1.507±0.73)μm]及D和E水源水最高剂量组[(1.111±0.53)、(1.225±0.64)μm]均高于阴性对照组[(0.530±0.22)μm],(P<0.05或P<0.01);体外微核试验计算微核率(‰)发现B、D、E出厂水和E水源水3个高剂量组[B:(32.667±11.02)、(30.00±10.00)、(26.833±10.17);D:(31.833±5.19)、(30.167±5.71)、(26.667±5.50);E出厂水:(34.00±7.40)、(31.00±7.46)、(27.50±8.64);E水源水:(30.00±9.88)、(29.50±9.71)、(25.50±9.73)]及D水源水前2个高剂量组[(27.167±6.59)、(25.667±7.03)]均高于阴性对照组(14.833±7.70),(P<0.05或P<0.01)。结论福州市生活饮用水水源水和出厂水受到一定程度的有机物污染。
Objective To evaluate the genotoxicity of drinking water in Fuzhou city in vitro.Methods Five districts and a water plant in each district were randomly selected in Fuzhou city.Two hundreds liters drinking water and source water were sampled in a water plant.XAD-2 resin was used to extract the organic matter in the water sample,and single cell gel electrophoresis assay and micronucleus test in vitro were used to detect the genotoxicity of organic extraction.Results The Olive tail-moment for drinking water samples（2,1,0.5 liter） with high contents of organic extraction from water plant B（1.321±0.5,1.310±0.82,1.209±0.48 μm）,D（1.297±0.48,1.289±0.76,1.235±0.69 μm）,E（1.553±0.65,1.534±0.68,1.507±0.73 μm） and source water samples（2 liters） from water plant D（1.111±0.53 μm）,E（1.225±0.64 μm） were significantly higher than that of negative control（0.530±0.22 μm,P0.05 or P0.01）.The micronucleus rate for drinking water samples（2,1,0.5 liter） with high contents of organic extraction from water plant B（32.67±11.02,30.00±10.00,26.833±10.17%）,D（31.833±5.19,30.17±5.71,26.667±5.50%）,E（34.00±7.40,31.00±7.46,27.50±8.64%）,and source water samples（2,1,0.5 liter） from water plant E（30.00±9.88,29.50±9.71,25.50±9.73%）,D（27.17±6.59,25.667±7.03%） were significantly higher than that of negative control（14.833±7.70%,P0.05 or P0.01）.Conclusion The drinking water and source water of Fuzhou city were polluted by organic compounds.
出处
《中国公共卫生》
CAS
CSCD
北大核心
2011年第2期129-130,共2页
Chinese Journal of Public Health
基金
福州市科学计划项目(2008-G-119)
