摘要
目的探讨儿童氟斑牙与维生素D受体(VDR)FokⅠ基因多态性的关系。方法选取河南省开封、通许2县8～12岁儿童高氟区143人和对照区94人为对象,按照氟斑牙患病情况分为高氟区患者组,高氟区非患者组及对照组;采用氟离子选择电极法检测尿氟含量,利用多聚酶链反应-限制性片段长度多态性(PCR-RFLP)技术分析雌激素受体(VDR)FokⅠ基因多态性。结果高氟区儿童氟斑牙患病率为51.7%,对照区未检出氟斑牙患者;高氟区VDR FokⅠ基因型分布频率氟斑牙患者为FF 32.4%(24/74),Ff 45.9%(34/74),ff 21.6%(16/74);非患者为FF40.6%(28/69),Ff 36.2%(25/69),ff 23.2%(16/69),对照区儿童为FF 31.9%(30/94),Ff 50.0%(47/94),ff 18.1%(17/94);3组儿童基因型频率分布差异无统计学意义(P>0.05);VDR FokⅠ基因型在高氟区不同性别儿童中分布相同,差异无统计学意义(P>0.05);VDR FokⅠ基因型在尿氟超标的氟斑牙患者和非患者中分布相同,差异无统计学意义(P>0.05)。结论高氟区儿童氟斑牙发生与VDR FokⅠ基因型无明显关系。
Objective To explore the distribution of VDR FokⅠ genotype in children who 1ived in areas with or without high fluoride,and to evaluate the relationship between children′s dental fluorosis and VDR FokⅠgene polymorphism.Methods Children aged 8 to 12 years,born and raised in high fluoride areas and contro1 areas in two counties of Henan province were recruited.They were divided into three groups according to dental fluorosis status：dental fluorosis group,non-dental fluorosis group from high fluoride area,and contorl group.The FokⅠmarker of VDR gene was genotyped using PCR-RFLP procedure.Fluoride levels in urine samples from the three groups were detected by fluoride ion selective electrode method.Results The prevalence rate of dental fluorosis in high fluoride areas was 51.7%.No dental fluorosis case was found in control areas.The frequency distribution of VDR FokⅠgenotype was FF 32.4%（24/74）,Ff 45.9%（34/74）,ff 21.6%（16/74）in children with dental fluorosis,FF 40.6%（28/69）,Ff 36.2%（25/69）,ff 23.2%（16/69）in children without dental fluorosis from high fluoride areas,and FF 31.9%（30/94）,Ff 50.0%（47/94）,ff 18.1%（17/94）in the children from contro1 areas,respectively.There was no significant difference among the three groups（P0.05）.Conclusion There was no correlation between dental fluorosis and VDR FokⅠpolymorphism.
出处
《中国公共卫生》
CAS
CSCD
北大核心
2011年第2期172-174,共3页
Chinese Journal of Public Health
基金
国家自然科学基金(81072247)
河南省自然科学基金(102300410073)
关键词
氟斑牙
基因多态性
维生素D受体
dental fluorosis
gene polymorphism
vitamin D receptor
