摘要
目的:建立测定赤芍总苷原料中芍药苷和赤芍总苷含量的方法。方法:采用反相高效液相色谱法。色谱柱为YMC-PackODS-A(250mm×4.6mm,5μm),流动相为乙腈-0.02mo·lL-1磷酸二氢钠(20∶80),检测波长分别为230、227nm。结果:芍药苷与赤芍总苷(以苯甲酸计)的进样量分别在0.832～4.160μg(r=0.9993)、0.0224～0.1120μg(r=0.9991)范围内与各自峰面积积分值呈良好线性关系;平均回收率分别为104.2%、105.2%,RSD分别为1.96%、1.94%(n均为9)。结论:本方法简便、易行,精密度、重复性、稳定性好,适用于赤芍总苷原料的分析测定。
OBJECTIVE： To establish determination methods of peoniflorin and total paeony glycoside in paeonia materials. METHODS： The content of peoniflorin and total paeony glycoside were determined by RP-HPLC. The determination was performed on YMC-Pack ODS-A （250 mm×4.6 mm, 5μm） with mobile phase consisted of acetonitrile-0.02 mol·L^-1 NaH2PO4（20： 80） at detection wavelength of 230nm and 227 nm. RESULTS： The linear range of peoniflorin and total paeony glycoside were 0.832-4.160 μg（r=0.999 3）and 0.022 4-0.112 0 p.g （r=0.999 1）, respectively. The average recoveries were 104.2% and 105.2% respectively. RSD were 1.96% and 1.94% （n=9）. CONCLUSION： The method is simple and easy for determination and analysis of paeonia materials. The method is characterized with the features of accuracy, reproducibility and stability.
出处
《中国药房》
CAS
CSCD
北大核心
2011年第7期623-625,共3页
China Pharmacy
基金
新疆农业大学教学研究项目(200927)
