摘要
微生物的存在会改变发酵液的电物性，发酵液在无线电频率范围内的电特性可用电容率和电导率充分地描述。实验证实发酵液在无线电频率范围内的电容率增量是测量频率和生物量浓度的函数。基于对发酵液电容率分布的研究，本文提出了测量生物量浓度的新方法。此方法不用取样就能对发酵液中的生物量进行实时在线测量，而且测得的是活的酵母浓度。本文制作的电极直接插入发酵器中并满足高温蒸汽灭菌条件。本方法在生化制药、食品发酵、啤酒酿造、污水检测等工业领域里有很好的推广应用前景。
This paper concerns a device and a method which monitor ydast concentrations on-line and in-situ in a fermentation process The presence of microbial cells would affect the dielectrical properties of microbial suspensions .At radio -frequencies the intrinsic passive electrical properties of cell suspensions are completely specified by its conductivity and permittivity,At radio -frequencies the dielectric permittivity of cell suspensions are monotonic function of the measuring frequency and cell density. Based on this understanding a new method for measuringbiomass concentration is proposed .Using this method the biomass concentrations are measured on-line and in-situ without taking samples from the bioreactor, and only viable cells are detected.The electrode which we developed could be directly inserted into the fermentor and could be sterilized in place.The method has wide use in the industrial field of pharmacy. brewery, sewage disposal.
出处
《食品科学》
EI
CAS
CSCD
北大核心
1999年第10期27-32,共6页
Food Science
关键词
发酵液
酵母浓度
实时在线检测
测定
酿酒
Cell suspension, Yeast density, On-line and in-situ monitoring, Permittivity, Sterilizing in place
