摘要
为研制牛传染性鼻气管炎病毒(IBRV)灭活疫苗,本研究以国内分离鉴定的IBRV LN01/08株为种毒,优化病毒增殖条件,获得病毒滴度达108.0 TCID50/mL,将其灭活制备疫苗。为比较不同免疫佐剂的效果,分别以矿物质白油和Montanide ISA206佐剂配制灭活疫苗,进行牛体免疫试验。临床观察和血清中和抗体检测结果表明,Montanide ISA206佐剂乳化的疫苗在降低副反应和增强免疫效果方面优于矿物质白油佐剂。应用MontanideISA206佐剂制备3批灭活疫苗,并对其安全性和免疫保护效果进行测定。结果表明该疫苗安全可靠,对强毒攻击可产生较好的抵抗力,攻毒保护率达80%。疫苗在2℃～8℃保存12个月后仍能保持良好的免疫效果。
Infectious bovine rhinotracheitis virus （IBRV） inactivated vaccine was prepared with IBRV LN01/08 strain. The high virus titer of 108.0 TCID50/mL was achieved by optimised replication conditions and emulsificated with amineral oil adjuvant and Montanide ISA206 adjuvant, respectively. The evaluation results showed that IBRV inactived vaccine with Montanide ISA206 adjuvant stimulated better immune response and caused less side-effects in comparison with mineral oil adjuvant in calves. The tests of three batches of the IBRV inactivated vaccine prepared with Montanide ISA206 adjuvant demonstrated that the vaccine could provide 80% protection for calves against the IBRV challenge. Furthermore, the study also indicated that the vaccine still had good immune effect for 12 month storage at 4 ℃.
出处
《中国预防兽医学报》
CAS
CSCD
北大核心
2011年第1期54-57,61,共5页
Chinese Journal of Preventive Veterinary Medicine
基金
农业公益性行业科研专项(200803018)
关键词
牛传染性鼻气管炎病毒
灭活疫苗
免疫佐剂
infectious bovine rhinotracheitis virus
inactivated vaccine
adjuvant
