摘要
目的:观察电针对脑梗死大鼠脑神经可塑性的影响,探讨其作用机制。方法:将60只大鼠随机分为假手术组(A组)、针刺组(B组)和非针刺组(C组),每组大鼠再随机分为1d、7d和14d共9个亚组。用线栓法制备脑梗死模型。针刺组大鼠进行电针刺激"足三里"和"内关"治疗,每日1次,每次20min;非针刺组和假手术组在相同时间予以捆绑固定,不进行针刺治疗。采用神经功能损伤评分(NSS)来评价神经功能缺损情况,免疫组化法测定各组大鼠脑梗死区周围神经生长相关蛋白-43(GAP-43)表达的变化情况。结果:1d时大鼠脑组织梗死区周围GAP-43的表达3组之间没有明显差别(P>0.05);7d和14d时,针刺组GAP-43阳性细胞表达的累积光密度值(IOD)值分别为8.990 1±0.0987、5.8161±0.2046,均明显高于假手术组的1.300 2±0.0933、1.3626±0.2166和非针刺组的2.7534±0.0875、1.6165±0.1868(均P<0.01)。结论:电针刺激"足三里"和"内关"能够改善脑梗死大鼠神经功能,促进其神经功能重塑,其机制可能与增加脑梗死大鼠脑梗死区GAP-43表达水平有关。
Objective To observe the effect of electroacupuncture on the neural plasticity in rats with cerebral infarction and investigate its mechanism. Methods Sixty rats were randomly divided into a sham operation group, an acupuncture group and a non-acupuncture group, and each group was randomly divided into a i-clay subgroup, a 7 day subgroup and a 14-day subgroup. Cerebral infarction model was induced by the thread embolism method. The rats in the acupuncture group were treated by electroacupuncture at ＂Zusanli＂ （ST 36） and ＂Neiguan＂ （PC 6） for 20 minutes, once each day, while the rats in the sham operation group and the non acupuncture group were just bound and fixed at the same time without acupuncture treatment. Neurological defects were assessed by Neurological Severity Score （ASS） and the changes of the expression of growth associated protein 43 （GAP-43） in peripheral nerve around cerebral infarction area were detected by immunohistochemistry technique. Results The expression of the positive ceils of GAP-43 around cerebral infarction area showed no significant distinction among the three groups at 1st day （P〉0.05）, while the GAP-43 expression level of around cerebral infarction area in the acupuncture group （IOD： 8. 990 1±0. 098 7,5. 816 1±0. 204 6） were significant higher than those in the sham operation group （IOD： 1. 300 2±0. 093 3,1. 362 6±0. 216 6） and in the non-acupuncture group （IOD： 2. 753 4±0. 087 5 ,1. 616 5±0. 186 8） at 7th day and 14th day, respectively （all P〈0.01）. Conclusion Electroacupuncture at ＂Zusanli＂ （ST 36） and ＂Neiguan＂ （PC 6） can improve the neural function and promote its remodeling in rats with cerebral infarction and the relevant mechanisms may be involved in enhancement of GAP-43 expressions around ischemic region.
出处
《中国针灸》
CAS
CSCD
北大核心
2011年第1期55-59,共5页
Chinese Acupuncture & Moxibustion
基金
广西卫生厅立项课题:桂卫Z2001011
广西大型仪器协作共用网资助:212-2005-05
关键词
电针
脑梗死
穴
足三里
穴
内关
神经生长相关蛋白-43
Eleetroacupuneturel Brain Infaretionl Point ST 36 （Zusanli）
Point PC 6 （Neiguan）
Grouth Associated Protein-43 （GAP-43）
