摘要
为了研究外源激素GA3和IAA对3个转基因新疆雪莲类黄酮高产毛状根系C17、C27、C46的植株再生及其总黄酮含量的影响,在培养基中添加不同浓度的GA3和IAA,结果发现,GA3浓度高于1.0 mg/L时,可诱导毛状根系产生不定芽,其中以GA3浓度为2.0 mg/L时,转基因毛状根系C17的不定芽再生率最高,可达82%。高压液相色谱以及紫外分光光度法测定结果表明,与未用激素处理的毛状根和它的再生植株相比,外源激素GA3和IAA能显著提高毛状根培养物中芹菜素和总黄酮的含量。毛状根系的组织干重与类黄酮的含量没有相关性,但毛状根系的再生率与类黄酮的含量几乎呈反相关性。
We investigated the plant regeneration and production of flavonoids in three high-yield flavonods transgenic Saussurea involucrata hairy roots C17,C27 and C46 by quantification of two phytohormones GA3 and IAA.The results showed that GA3 concentration at more than 1.0 mg/L could induce adventitious shoots in the hairy root lines.The highest shoot regeneration rate,about 82%,was obtained when the hairy roots C17 were cultured with 2.0 mg/L GA3.The results on HPLC and UV spectrophotometry showed that exogenous application of both GA3 and IAA increased the content of flavonoids in the hairy roots.The contents of flavonoids and apigenin in the hormone-treated hariy roots and regenerates were higher comparing with those in the untreated hairy roots and the regenerates.However,the content of flavonoids was not related to tissue weight,and was negatively related to the regeneration efficiency.
出处
《生物工程学报》
CAS
CSCD
北大核心
2011年第1期69-75,共7页
Chinese Journal of Biotechnology
基金
国家自然科学基金(No.30472158)资助~~
关键词
新疆雪莲
植物激素
植株再生
毛状根
类黄酮
Saussurea involucrata
phytohormones
plant regeneration
hairy root
flavonoids
