摘要
目的:研究健康受试者口服苦寒方剂黄连解毒汤前后尿液中的生物标志物及其变化,探讨黄连解毒汤对健康人群代谢的影响。方法:10名健康受试者自愿参与本试验,签署知情同意书,口服黄连解毒汤3d,分别于给药前和给药后的每天早晨采集尿液样品,在4℃下13000×g离心10min,移取上清液于-75℃超低温冰箱中冷冻保存。以超高效液相-四极杆-飞行时间/质谱为核心技术,以主成分分析和偏最小二乘判别分析为数据解析手段,研究口服黄连解毒汤前后健康人群尿液中代谢物组时量的轨迹变化和对应关系。结果:黄连解毒汤的给予使得尿液代谢物组发生了明显的变化,对健康人群的代谢产生了明显的影响;经模式识别分析,初步检识7个被认为能够表征黄连解毒汤作用于健康人群的潜在生物标志物,鉴定了1个具有生物学意义的生物标志物(2-甲酰氨基-苯甲酸)。结论:从生物体内代谢层面探讨黄连解毒汤对健康人群尿液代谢物组的影响,为其作用于热证患者的性效关系研究提供了理论基础和技术支持。
Objective：To study biomarkers and their diversification in healthy people’s urine before and after oral administration of Huanglian Jiedu Decoction,a compound Chinese herbal medicine,and to investigate the influence of Huanglian Jiedu Decoction on urine metabonomics of healthy people.Methods：Ten healthy volunteers joined in this experiment and signed informed consent. They were given oral administration of Huanglian Jiedu Decoction for 3 days,and their urine was collected every morning. The samples were centrifuged at 13 000 ×g for 10 min at 4 ℃,and the supernatant was stored at-75 ℃. The samples were examined by a core technology called ultra-performance liquid chromatography quadruple time of flight/mass spectrometry and then the data were analyzed by principal components analysis and partial least square-discriminant analysis to investigate trajectory diversification and corresponding relations of the time and amount of urine metabonomics before and after oral administration of Huanglian Jiedu Decoction.Results：Evident variance of urine metabonomics took place after oral administration of Huanglian Jiedu Decoction,which showed that the metabolism of healthy people had been visibly affected. Seven potential biomarkers which play a role in healthy people on behalf of Huanglian Jiedu Decoction were preliminarily found,and one biomarker which had biological significance was identified as 2-（formylamino）-benzoic acid.Conclusion：The effects of Huanglian Jiedu Decoction on urine metabonomics in healthy people investigated at the level of metabolism can provide theoretical and technical support for the study of Huanglian Jiedu Decoction’s influence on heat syndrome
出处
《中西医结合学报》
CAS
2011年第1期77-83,共7页
Journal of Chinese Integrative Medicine
基金
国家重点基础研究发展计划(973计划)资助项目(No .2007CB512608)
关键词
中草药
黄连解毒汤
健康人群
尿
代谢组学
生物标志物
药理学
drugs, Chinese herbal
Huanglian Jiedu Decoction
healthy people
urine
metabonomics
biomarkers,pharmacological
