摘要
目的 观察磁刺激对脊髓损伤（SCI）后SD大鼠的神经细胞凋亡及诱导型一氧化氮合酶（iNOS）生成的影响.方法 雄性SD大鼠32只,分为磁刺激组16只,对照组16只,每组又分为SCI后第6小时,第12小时,第24小时和第72小时4个时间点,每个时间点4只.2组采用脊髓横切法造成SCI模型,磁刺激组在每个时间点给予磁刺激后即处死,对照组在伤后相应时间点处死.用脱氧核糖核苷酸末端转移酶介导的缺口末端标记法标记凋亡细胞,用免疫组化法检测诱导型一氧化氮合酶阳性细胞.观察各时间点的凋亡指数和诱导型一氧化氮合酶阳性细胞率.结果 凋亡细胞在伤后第6小时即出现在脊髓组织中,第24小时与第72小时凋亡细胞数显著增加;iNOS阳性细胞在SCI后第6小时和第12小时时间点几乎没有表达,从第24小时到第72小时显著升高.与对照组相比,在伤后第6小时和第12小时,磁刺激组的凋亡指数末见明显减少（P＞0.05）;但是在第24小时和第72小时的时间点上,磁刺激组的凋亡阳性细胞显著少于对照组（P＜0.01）.诱导型一氧化氮合酶阳性细胞率两组在各个时间点上的差异无统计学意义（P＞0.05）.结论 磁刺激可以降低SCI后神经细胞的继发性损伤,抑制神经细胞的凋亡,对SCI早期的神经细胞具有保护作用,然而其机制是否通过对诱导型一氧化氮合酶表达的抑制而实现的需要进一步研究.
Objective To investigate effects of magnetic stimulation on apoptosis of nerve cells and the production of inducible nitric oxidate synthase （iNOS） after spinal cord injury （SCI）. Methods Thirty-two SpragueDawley male rats were randomly divided into a magnetic stimulation group （n = 16） and a control group （n = 16）.SCI models were established by spinal cord transection in both groups. Rats were sacrificed at the 6th, 12th, 24th and 72nd hour post-injury, but the rats in the stimulation group received magnetic stimulation before being sacrificed.Apoptosis index （AI） and iNOS-positive cells rate were recorded at each time point. Results Apoptotic cells could be observed by the 6th hour post-injury, and were elevated from the 24th to the 72th hour. iNOS-positive cells were few at the first two time points, but had increased significantly at the 24th and 72nd hour post-injury. Compared with the control group, the apoptosis index of the stimulation group decreased a little at the 6th and 12th hour, but not significantly. The difference was quite significant at the 24th and 72nd hour, however, and the AI in the stimulation group decreased much more than that in the control group. There was little difference in the rate of iNOS-positive cells between the control and stimulation groups at any time point. Conclusions Magnetic stimulation could inhibit neural apoptosis and protect neurons from secondary SCI, but it has little effect on iNOS production.
出处
《中华物理医学与康复杂志》
CAS
CSCD
北大核心
2011年第1期10-13,共4页
Chinese Journal of Physical Medicine and Rehabilitation
关键词
脊髓损伤
磁刺激
凋亡
诱导型一氧化氮合酶
Spinal cord injury
Magnetic stimulation
Apoptosis
Nitric oxldate synthase
