摘要
目的:研究表皮生长因子(epidermalgrowthfactor,EGF)在小鼠早期胚胎体外发育中的作用,以及EGF联合胰岛素样生长因子-Ⅱ(insulin-likegrowthfactor-II,IGF-Ⅱ)对小鼠早期胚胎体外发育是否有协同促进作用。方法:①在mKSOM培养液中添加0ng/ml(对照组)、0.1ng/ml、1ng/ml、10ng/ml、100ng/ml的EGF、10ng/mlIGF-Ⅱ、1ng/mlEGF+1ng/mlIGF-Ⅱ、1ng/mlEGF+10ng/mlIGF-Ⅱ、10ng/mlEGF+1ng/mlIGF-Ⅱ、10ng/mlEGF+10ng/mlIGF-Ⅱ培养小鼠1-细胞胚胎,每组胚胎在培养箱中连续培养120h,每24h观察胚胎发育情况,分别计算2-细胞率、4-细胞率、桑椹胚率、囊胚率和孵化率,并进行囊胚细胞计数。结果:添加1ng/ml、10ng/mlEGF组的囊胚率及孵化率显著增加;EGF+IGF-Ⅱ组较单一添加组、对照组囊胚率及孵化率显著增加(P<0.05),且EGF+IGF-Ⅱ组囊胚细胞计数显著高于其他组(P<0.05),其中10ng/mlEGF+10ng/mlIGF-Ⅱ组的孵化率及囊胚细胞数最高(P<0.05)。结论:EGF浓度在1￣10ng/ml范围内可以提高体外培养鼠胚的囊胚率及孵化率;EGF及IGF-Ⅱ对体外培养鼠胚的发育有协同促进作用;本实验范围内10ng/mlEGF+10ng/mlIGF-Ⅱ为最优组合。
Objective：To study the effects of epidermal growth factor（EGF） and it combining insulin-like growth factor-Ⅱ（IGF-Ⅱ） on the development of 1-cell mouse embryos in vitro.Methods：The 1-cell embryos were cultured up to 120 h at 37℃ under 5% CO2 in a sealed culture chamber and observed every 24 h,determining the proportion of 2-cell,4-cell,morula,blastocyst,hatched blastocyst and counting the total cell numbers.Results：Adding 1 ng/ml,10 ng/ml EGF significantly increased the blastocyst rates and hatched blastocyst rates compared with the control（P0.05）.Adding 10 ng/ml EGF combined with 10 ng/ml IGF-Ⅱ significantly increased the blastocyst rate,hatched blastocyst rate and the total cell numbers（P0.05）.Conclusion：1） Among the range of the concentrations 1-10 ng/ml,the EGF could increase blastocyst rates and hatchedblastocyst rates of 1-cell mouse embryo in vitro.2） EGF and IGF-Ⅱ have coordinating advanced effect on the development of 1-cell mouse embryo.3） 10 ng/ml EGF combining 10 ng/ml IGF-Ⅱ is the best group.
出处
《生殖与避孕》
CAS
CSCD
北大核心
2011年第1期1-5,29,共6页
Reproduction and Contraception
基金
云南省教育厅科学研究基金项目
项目号是04Z843C
