摘要
目的:探讨氧化苦参碱(OM)对SW1990细胞侵袭力的影响.方法:取人胰腺癌细胞SW1990为研究对象,随机分为对照组和OM组,OM组再按照OM剂量不同,分为1,2,4g/L3组.用RT-PCR检测MMP-2mRNA表达变化,以细胞划痕试验和Transwell小室法检测SW1990细胞侵袭力和迁移的变化,用四甲基偶氮唑蓝(MTT)比色法检测OM对SW1990细胞增殖的影响作用.结果:RT-PCR显示MMP-2mRNA在SW1990细胞中的表达,OM各组较对照组显著降低(0.53±0.03,0.42±0.02,0.29±0.03vs0.70±0.03,均P<0.05).细胞侵袭力测定显示,OM各组侵袭过Matrigel胶的细胞数显著低于对照组(325.00±64.43,206.00±84.76,124.00±46.78vs498.00±78.54,均P<0.05),OM组细胞侵袭能力较对照组显著降低.细胞迁移实验结果显示,OM各组穿过滤膜的细胞数显著低于对照组(385.0±58.9,287.0±79.8,186.0±60.9vs586.0±85.8,均P<0.05).结论:OM可能通过下调MMP-2mRNA的表达,抑制胰腺癌细胞侵袭转移能力.
AIM： To examine the effects of treatment with oxymatrine （OM） on MMP-2 expression and cell invasion in human pancreatic carcinoma cell line SW1990. METHODS： Cultured SW1990 cells were randomly divided into two groups： control groupand OM group. The OM group was further divided into three subgroups, which were treated with three different doses of OM （1, 2 and 4 g/L）. MMP-2 expression in SW1990 cells was analyzed by real-time RT–PCR. Cell invasion, migration and proliferation were measured by scratch assay, transwell invasion assay and MTT assay, respectively. RESULTS： The expression levels of MMP-2 mRNA in SW1990 cells in the three OM sub- groups were significantly lower than that in the control group （0.53 ± 0.03, 0.42 ± 0.02, 0.29 ± 0.03 vs 0.70 ± 0.03, all P 0.05）. The invasion of cells in the three OM subgroups was markedly lower than that in the control group （325 ± 64.43, 206 ± 84.76, 124 ± 46.78 vs 498 ± 78.54, all P 0.05）. The mobility of cells in the three OM subgroups was also significantly lower than that in the control group （385.0 ± 58.9, 287.0 ± 79.8, 186.0 ± 60.9 vs 586.0 ± 85.8, all P 0.05）. CONCLUSION： OM reduces the invasion of SW1990 cells possibly by inhibiting the expression of MMP-2.
出处
《世界华人消化杂志》
CAS
北大核心
2011年第1期19-24,共6页
World Chinese Journal of Digestology
基金
武警部队科研基金资助项目
No.WKH2006-9
武警医学院科研基金资助项目
No.WY2008-13~~
关键词
胰腺癌
肿瘤侵袭
氧化苦参碱
基质金属蛋白酶
Pancreatic carcinoma
Tumor invasion
Oxymatrine
Matrix metalloproteinase
