摘要
目的:研究ARHI基因在胃癌组织与癌旁正常组织中的表达差异及其与患者临床病理特征的相关性.方法:用免疫组织化学SP法检测胃癌组织与癌旁正常组织中ARHI基因编码蛋白的表达情况,用SPSS13.0统计软件对ARHI蛋白的表达差异及其与患者的临床病理特征进行统计学分析.结果:ARHI蛋白在试验组中阳性表达27例,阴性表达35例,表达缺失率56.45%;对照组中阳性表达21例,阴性表达7例,表达缺失率25%,组间比较有明显统计学差异(P<0.01).在试验组中,ARHI蛋白表达与患者的年龄、性别、发生部位、肿瘤大小、大体类型、是否淋巴结转移及神经、脉管侵犯均无明显统计学差异,而与肿瘤的分化程度及TNM分期有关(P<0.05).结论:ARHI蛋白在胃癌组织中表达明显下调或缺失,而在胃正常组织中普遍表达.ARHI蛋白表达与患者肿瘤的分化程度及TNM分期有关.
AIM： To investigate the expression of aplasia ras homologue member I （ARHI）, a tumor suppressor gene, in gastric cancer and to define the correlation between ARHI expression and the clinicopathological characteristics of gastric cancer. METHODS： Immunohistochemistry was used to detect the expression of ARHI protein in 62 gastric cancer specimens and 28 tumor-adjacent tissue specimens. The correlation between ARHI protein expression and clinicopathological characteristics of gastric cancer was then analyzed.RESULTS： The percentage of gastric cancer specimens negative for ARHI staining was significantly higher than that of tumor-adjacent tissue specimens （56.45% vs 25.0%, P 0.01）. The expression of ARHI protein was not associated with age, gender, tumor location, tumor size, or metastasis in patients with gastric cancer. However, ARHI protein expression was correlated with tumor differentiation and TNM stage in patients with gastric cancer （both P 0.05）. CONCLUSION： The expression of ARHI protein is significantly reduced or absent in gastric cancer. ARHI protein expression is closely related with tumor differentiation and TNM stage in patients with gastric cancer.
出处
《世界华人消化杂志》
CAS
北大核心
2011年第1期25-30,共6页
World Chinese Journal of Digestology
基金
福建省厦门市卫生局重点基金资助项目
No.3502z20077038~~
