摘要
目的研究呼和浩特地区2008年6月～2009年5月婴幼儿腹泻轮状病毒(rotavirus,RV)的基因型。方法采集内蒙古妇幼保健院2008年6月～2009年5月所有≤59月龄的住院腹泻患儿粪便标本313份。采用酶联免疫吸附试验(ELISA)检测RV;对RV抗原阳性的标本用巢式逆转录聚合酶链式反应(nested RT-PCR)对6种流行的VP7基因型(G1、G2、G3、G4、G8、G9)和5种流行的VP4基因型(P[8]、P[4]、P[6]、P[9]、P[10])进行分型;最后整理数据,应用SPSS 13.0统计软件包进行统计分析。结果 313份粪便标本中,125份为RV抗原阳性(39.94%)。对阳性标本进行VP7基因分型,结果为G1型64.8%(81/125)、G2型2.4%(3/125)、G3型14.4%(18/125)、G9型0.8%(1/125)、G1+G3混合型16.8%(21/125)、G2+G3混合型0.8%(1/125);进行VP4基因分型,结果为P[8]型84.0%(105/125)、P[4]型4.0%(5/125)、P[8]+P[4]型1.6%(2/125),13份未分出型别。结论 RV是引起呼和浩特地区婴幼儿腹泻的主要病原之一,主要流行株为G1P[8]型,首次在内蒙地区检出G9型感染。
Objective To study the epidemiological characteristics of infantile diarrhea with rotavirus in Hohhot from June 2008 to May 2009. Methods A total of 313 stool specimens were collected from children under 5 years old with acute diarrhea who were hospitalized in Inner Mongolia Maternal and Child Health Hospital from June 2008 to May 2009.Enzyme linked immunosorbent assay（ELISA） was used to detect rotavirus genemic RNA.Nested reverse transcription polymerase chain reaction（nested RT-PCR） was performed to identify rotavirus VP7（G1、G2、G3、G4、G8、G9） and VP4（P[8]、P[4]、P[6]、P[9]、P） genotypes.SPSS 13.0 were applied for data analysis.Results 313 stool specimens,125 were detected rotavirus positive, the infection rate was 39.94%.Among 125 positive samples G1（64.8%） was the most predominants train,followed by G1＋G3（16.8%）,G3（14.4%）,G2（2.4%）,G9（0.8%） and G1＋G2（0.8%）;P genotyping showed P[8]（84.0%） was the most common,followed by P[4]（4.0%）,P[8]＋P[4]（1.6%）,13 strains were nontypeable.Conclusions Rotavirus were one of the most common cause of acute gastroenteritis among infants in Huhhot.G1P[8] was the main prevalent serotype.For the first time in Inner Mongolia region,G9 serotype was detected.
出处
《中华疾病控制杂志》
CAS
2011年第1期52-55,共4页
Chinese Journal of Disease Control & Prevention
