摘要
随着法医DNA分析技术的发展,越来越多低模板量DNA的生物检材被提取,并要求进行STR分型。在此对快速发展的各种低模板量DNA STR分型方法如增加聚合酶链反应(PCR)循环数、纯化PCR扩增产物、激光捕获显微切割和PCR扩增前的全基因组扩增等予以介绍,并就LT-DNA法庭科学应用中存在的相关问题予以论述。
More and more biological evidences known as low template DNA,for the typing of STRs by using the typical commercial kit formats,are consistently detected in forensic DNA analysis.There are a number of methodologies to enable LT-DNA typing,such as the addition of PCR numbers,purification of PCR products,laser capture microdissection,and whole genome amplification.
出处
《医学综述》
2011年第2期167-169,共3页
Medical Recapitulate
