摘要
目的探讨α-L-岩藻糖苷酶(AFU)、癌胚抗原(CEA)、糖抗原CA199、恶性肿瘤相关物质(TSGF)联合检测对甲胎蛋白(AFP)阴性肝癌诊断的互补作用。方法选取58例AFP阴性肝癌患者,运用酶联免疫吸附技术检测CEA、CA199,生化定量分析法检测AFU、TSGF;采用ROC曲线评价4种肿瘤标志物(TM)实验的敏感性和特异性。结果 4种TM单项检测阳性率分别为AFU(64%)、CEA(28%)、CA199(22%)、TSGF(66%),联合检测阳性率为93%,联合与单项检测的阳性率相比均有显著性差异(P均<0.05);4种TM检出12种阳性组合方式,各种组合系列和联合检测的阳性率比较均有显著性差异(P均<0.05);4种TM的ROC曲线下面积都在50%参考线以上,实验的敏感性和特异性综合性能较好。结论 4种TM联合检测对AFP阴性肝癌的诊断有较好的互补作用和临床应用价值。
Objective It is to observe the complementary role on combination detection of AFU, CEA, CA199, TSGF in the diagnosis of AFP - negative hepatoma. Methods 58 patients with AFP - negative were selected, then the levels of CEA, CA199 of them were detected by ELISA assay, and the levels of AFU and TSGF were detected by quantitave biochemical analysis ; the sensitivity and specificity of four kings of tumor markers were evaluated by ROC curve. Results The positive rates of AFU, CEA, CA199 and TSGF were 64%, 28% , 22% and 66% respectively, the positive rate of combined detection was 93% , there were significant differences between the individual and combined detection, （P 〈 0. 05 ）; four kinds of tumor markers checked out 12 kinds of positive associations, there were significant differences between combined detection every association and series detection （P 〈 0. 05 ） ; the areas under ROC curve were all above 50% reference line in four kinds of tumor markers, the sensitivity and specificity of four kings of tumor markers were satisfactorily. Conclusion Four kinds of tumor markers combined detection has a good complementary diagnostic and clinical application value in the diagnosis of AFP - negative hepatoma.
出处
《现代中西医结合杂志》
CAS
2011年第6期660-662,共3页
Modern Journal of Integrated Traditional Chinese and Western Medicine
关键词
肿瘤标志物
肝癌
AFP阴性
联合检测
tumor markers
hepatoma
AFP - negative
combination detection
