摘要
目的研究肝硬化门静脉高压患者胃左静脉、脾静脉壁标本的一氧化氮合酶(NOS)、血管内皮生长因子(VEGF)的表达,探讨肝硬化发生机制。方法 34例肝硬化门静脉高压症患者采用手术治疗,30例患者伴严重消化道出血或脾脏功能亢进,采用胃左静脉断流、脾脏切除,4例脾脏增大伴脾脏功能亢进患者单纯脾脏切除,取冠状静脉、脾静脉血管壁(0.5mm×0.5mm)SP法测定,观察NOS、VEGF染色表达情况。结果脾门血管壁免疫组化VEGF表达情况:14例(41.18%)强阳性(++),10例(29.41%)阳性(+),10例(29.41%)弱阳性或阴性(±或-);NOS表达情况:20例(58.8%)脾门血管表达阳性,14例(41.18%)阴性。30例胃左断流手术标本中,胃左静脉VEGF表达:14(46.67%)例强阳性(++),8例(26.67%)阳性(+),8例(26.67%)为弱阳性或阴性(±或-)。14例(46.67%)胃左血管内皮eNOS表达阳性。结论门静脉高压时门静脉系统血管内皮NOS、VEGF呈高表达,证明肝硬化门静脉高压时血管壁结构发生异常改变。
Objective To investigate the NOS,VEGF expression in endothelium of spleen and left gastric vein in patients with portal hypertension caused by cirrhosis.Methods 34 patients with portal hypertension were enrolled in the study,The expression of NOS and VEGF in endothelium of left gastric vein and spleen vein was determined by immunohistochemistry.Results The expression of VEGF in the endothelium of spleen venous was strongly positive in 14 cases（41.18%）and moderately positive in 10 cases（26.47%）,which was negative in 10 cases（26.47%）,however,which in left gastric vein was strongly positive in 14 cases（46.67%） and moderately positive in 8 cases（26.67%）and negative in 8 out of 30 cases（26.67%）.The expression of eNOS in the endothelium of spleen vein was positive in 20 cases（20/34,52.94%）and 14 cases（14/30,40.67%）in left gastric vein.Conclusion Both NOS and VEGF are highly expressed in the endothelium of portal vein,which suggest that the changes of vein structure may play an important role in portal hypertension.
出处
《河北医药》
CAS
2011年第2期174-176,共3页
Hebei Medical Journal
