摘要
目的：在裸鼠活体水平上观察ｂｃｌ － ２ 反义寡聚核苷酸（ａｎｔｉｓｅｎｓｅ ｏｌｉｇｏｄｅｏｘｙｎｕｃｌｅｏｔｉｄｅ ， ＡＳＯＮ） 对鼻咽癌细胞株ＣＮＥ２ － ｂａｘα凋亡的影响。方法：ＤＮＡ 合成仪合成２２ｎｔ ＡＳＯＮ 片段，ＲＴ－ ＰＣＲ 检测ｂｃｌ － ２ 表达，电镜观察细胞凋亡。结果：反义ｂｃｌ － ２ ＤＮＡ 片段局部注射ＣＮＥ２ － ｂａｘα致瘤组织，ＲＴ－ ＰＣＲ 检测肿瘤组织中ｂｃｌ － ２ 表达被封闭，电镜观察视野下可见大量癌细胞凋亡，癌细胞数目减少，细胞间组织大量纤维化；凋亡的癌细胞电子密度增加，具典型凋亡特征。反义ｂｃｌ － ２ 封闭１ 周，抑瘤率７４－４ ％ ，第２ 周为４６－０ ％ 。结论：反义ｂｃｌ－ ２ 抑制鼻咽癌细胞株ＣＮＥ２ － ｂａｘα致瘤性。
AIM: To observe the effect of bcl-2 antisense oligod eoxynucleotide (ASON) on nude mice habouring tumor by inoculated human nasopharyngeal carcinoma cell line CNE 2-baxα. METHOD: 22nt bcl-2 ASON was synthesized using DNA synthesizer. Apoptosis of neoplastic cells were observed with electron microscopy. RESULTS: Tumors were growing after CNE 2 baxα cells were subcutaneously inoculated in nude mice. Suppression of bcl-2 expression was verified with RT-PCR assay after bcl-2 ASON was injected into the tumors. We also identified by electron microscopy that after ASON treatment large amount of tumor cells underwent apoptosis and the number of tumor cells reduced. The electron density of apoptotic cells was increased and apoptotic bodies formed.7 days after antisense bcl-2 DNA injection, the tumor-inhibiting rate was 74.4%, 14 days after, the rate was 46.0%. CONCLUSION:Antisense bcl-2 DNA inhibits the CNE 2-baxα tumor growth and enhances apoptosis of CNE 2-baxα cells.
出处
《中国病理生理杂志》
CAS
CSCD
北大核心
1999年第8期714-716,共3页
Chinese Journal of Pathophysiology
基金
国家教委博士点基金
