摘要
采用逆转录ＰＣＲ技术自ＳＤ大鼠脾细胞中克隆出全长１１２５个ｂｐ的编码主要组织相容性抗原Ⅰ类分子（ＭＨＣ－Ⅰ）ｃＤＮＡ，经限制性内切酶图谱分析证实后，定向插入表达载体ｐＢＶ２２０，并筛选出带有插入片断的阳性克隆，为研究在原核或真核表达系统中表达及其在抗原识别、免疫应答中的作用奠定了基础。
The total RNA was extracted from the Rat Spleen cells. According to the published sequence of Rat MHC Ⅰ(rMHC Ⅰ), a couple of primers for rMHC Ⅰ was designed and synthesized.Through reverse trans cription and Polymerase chain reaction, obtained a specific band of rMHC ⅠcDNA fragment from Rat spleen cells. The obtained fragment was inserted to pBV220 plasmid and transformed to E. coli DH5a. Restriction enzyme digestion and PCR identified it as Rat MHC Ⅰ with the whole reading frame of 1125 base pairs.
出处
《免疫学杂志》
CAS
CSCD
北大核心
1999年第3期161-163,共3页
Immunological Journal
基金
中国博士后科学基金
