摘要
目的：探索黄苓中的放射损伤防护作用有效成分。方法：黄芩水提液酸化保温（８０℃）５０ 分钟，滤去析出的黄芩黄酮类（ Ｆ Ｓ Ｂ Ｇ） ，水溶液用经典分离法得一沉淀物（ Ⅰ） 、化学分析其结构类型；用６０ Ｃｏ ７ ．７ Ｇｙ ～８ ．７ Ｇｙ 全身照射纯系 Ｎ Ｉ Ｈ 雌性小鼠，照前３５ ～４５ ｍｉｎ 每鼠腹腔注射（ Ⅰ）１ｇ／ｋｇ 或２ｇ／ｋｇ ，观察生存防护效力、３０ 天存活率和照射４．０ Ｇｙ（２ｇ／ｋｇ） 后第４ 天和第１２ 天的 Ｗ Ｂ Ｃ、 Ｐ Ｌ Ｔ、 Ｒ Ｂ Ｃ 和 Ｈ Ｇ Ｂ。结果：（１） Ｆ Ｓ Ｂ Ｇ 和（ Ⅰ） 均对三氯化铁、 Ｍｏｌｉｓｃｈ 、 Ｆｅｈｌｉｎｇ 试剂呈阳性反应，前者对浓硫酸、碱试液和盐酸镁粉呈阳性而（ Ⅰ） 呈阴性；（２） 每鼠１ ｇ／ｋｇ 和２ ｇ／ｋｇ 剂量均显著提高生存防护效力（１ ．３５ ～２ ．０８ 治疗组３０ 天存活时间和对照组３０ 天存活时间之比值） 和３０ 天存率（ 治疗组为８．３３～４１ ．４６ ％ ， 对照组为０ ～２ ．５０ ％ ） ，２ ｇ／ ｋｇ 能明显增加４．０ Ｇｙ 照后第４ 天和第１２ 天的 Ｗ Ｂ Ｃ、 Ｐ Ｌ Ｔ、 Ｒ Ｂ Ｃ 和 Ｈ Ｇ Ｂ。结论：（１） 对浓硫酸等的呈色反应差异表明（ Ⅰ） 和 Ｆ Ｓ Ｂ Ｇ 化学结构不同；对
Objective:The effective principle with protective effect on radiation injury from Scutellaria baicalensis Georgi (SBG) was found and studied. Methods:The heated extracted aqueous liquid from SBG was acidified and kept hot for 50 minutes in 80℃,the flavonoids of SBG (FSBG) were precipitated and removed by filter.A precipitate (Ⅰ) from the filtered solution was obtained by the classical separation process.Tests were conducted on mice to detect whether (Ⅰ) is effective for protection against γ ray irradiation.Intraperitoneal injection(ip) of (Ⅰ) 1 g/kg and 2 g/kg in NIH female mice 35～45 minutes befores 7.7～8.7 Gy of whole body 60Co irradiation were conducted,the survival effects,30 days survival rate and in case of ip of (Ⅰ) 2 g /kg the WBC,PLT,RBC and HGB were observed on 4th and 12th day after 4.0 Gy exposure. Results:Positive reactions were shown with following chemical reagents:ferric chloride,Molisch,Fehling reagents on FSBG and (Ⅰ),however,concentrated sulfuric acid,alkaline reagents and hydrochloric acid Mg powder showed positive reaction on FSBG,but negative on (Ⅰ).Intraperitoneal injection of (Ⅰ) 1 g/kg and 2 g/kg increased the survival effects(1.35～2.08,the ratio of mean survival time in a treated group to that of the control group in 30 d after irradiation) and 30 days survival rate (8.33～41.46%in treated and 0～2.50%in control group,respectively) significantly in comparison with control mice.In cases of ip of (Ⅰ) 2 g/kg the WBC,PLT,RBC and HGb were all increased on 4 th and 12 th day after 4.0 Gy exposure as compared with control.Conclusions:1 The different coloration to chemical reagents (concentrated sulfuric acid and etc.) showed that there are different chemical structures between(Ⅰ) and FSBG.The positive reactions (ferric chloride and etc.) suggested that the main constituent of (Ⅰ) was a chemical type of glycoside with phenolic hydroxyl group. 2 The glycoside with phenolic hydroxyl group exhibited good radioprotective effect against whole body lethal irradiation of mice.3 The study may be considered as new application of SBG.
出处
《癌症》
SCIE
CAS
CSCD
北大核心
1999年第4期422-425,共4页
Chinese Journal of Cancer
基金
中山医科大学肿瘤防治中心资助
关键词
黄芩
酚性甙类
放射损伤
防护剂
小鼠
Scutellaria baicalensis Georgi
Glycoside with phenolic hydroxyl group
Protective agent on radiation injury mice
