摘要
目的研究纳米铜的肾细胞毒性,探讨纳米铜的溶出度在纳米铜致大鼠肾毒性中的作用。方法利用MTT法和LDH漏出率测定纳米铜的细胞毒性,利用一价铜特异性螯合剂、分光光度法、电感耦合等离子体-原子发射光谱法分析纳米铜粒子在培养体系中的溶出度。结果纳米铜可以剂量依赖性方式诱导细胞活力的下降,作用24 h对HK-2细胞的IC50为41.3μg.mL-1;纳米铜可以剂量依赖性方式引起细胞的LDH漏出增加,一价铜离子特异性螯合剂BCS可抑制纳米铜或氯化铜诱导的细胞毒性;在培养体系中,纳米铜粒子的溶解率呈现时间依赖性升高。结论纳米铜对肾细胞具有明显毒性作用;纳米铜在一定剂量范围内,转化为铜离子是其发挥细胞毒作用的重要方式。
OBJECTIVE To study the cytotoxicity of Nanocopper particales on HK-2 cell,and the relation between the dissolution rate of Nanocopper particales and the cytotoxicity.METHODS The cytotoxicity of Nanocopper particales on HK-2 cell were studied by measuring MTT and LDH.The dissolution rate of Nanocopper particales were measured by chelating agent,absorption spectrometry and inductively coupled plasma-atomic emission spectrometry methods.RESULTS Nanocopper particales can depress the growth dose dependent.The IC50 of Nanocopper particales to HK-2 cell was 41.3 μg·mL-1.Nanocopper particales can increased the leakage of LDH in HK-2 cell dose dependent.Chelating agent BCS can depress the cytotoxicity induced by Nanocopper particales or CuCl2.157.5 μmol·L-1 and 315 μmol·L-1 Nanocopper particales can increased the content of Cu2＋ time dependent.The dissolubility Nanocopper particales also increased time dependent.CONCLUSION At certain dose and exposure time,the cytotoxicity of Nanocopper particales on HK-2 cell is obvious.The transformation of Nanocopper particales to copper ions may play important roles in cytotoxicity.
出处
《华西药学杂志》
CAS
CSCD
北大核心
2011年第1期41-44,共4页
West China Journal of Pharmaceutical Sciences
关键词
纳米药物
纳米铜
肾毒性
溶出度
Nanodrugs
Nanocopper
Nephrotoxicity
Dissolution rate
