摘要
利用自花授粉后形成的花粉管通道将海岸耐盐植物红树总 Ｄ Ｎ Ａ 导入辣椒，其后代的耐盐性明显增强，在海滩上试种，用海水直接浇灌，约５５％ 的转化株能开花、结果，而对照株全部死亡。进行蛋白质 Ｓ Ｄ Ｓ Ｐ Ａ Ｇ Ｅ电泳和 Ｒ Ａ Ｐ Ｄ 分析，分别发现一条１７５ Ｋ Ｄ蛋白质和一条１１ Ｋｂ Ｄ Ｎ Ａ 的特异性谱带，表明通过花粉管通道导入外源 Ｄ Ｎ Ａ 是可行的。
Via the pollen tubes form ed after self pollination into Capsicum annuum cv, thetotal D N A of Rhizophora apoculata, a selt resistant,seashore grow n plant,w as introduced. Theprogenies w ere grow n on beach, and w atered directly w ith saline. There w as obviousim provem ent in the salt tolerance am ong the transform ed plants. Approxim ately 55% of thetransform ed plants could bloom and seed. How ever, none of the non transform ed controlsurvivedunder the pressure of saline. S D S P A G Eof protein and R A P Danalysis revealed a specific proteinband of 17.5 K D and a specific D N A band of 1.1kb, respectively. The results dem onstrated thatit is feasible to introduce exogenous D N A through pollen tubes, and the im proved salt toleranceof the progenies of transform ed plants is related to the genom ic changes.
出处
《生物工程进展》
CSCD
1999年第5期19-24,共6页
Progress in Biotechnology
基金
国家重点科技攻关项目
关键词
外源DNA导入
耐盐
RAPD
辣椒
introduce, exogenous D N A, salt tolerance, S D S P A G E, R A P D, Capsicumannuum cv
