摘要
目的:研究铜绿假单胞菌多重耐药情况和相关机制。方法:采用聚合酶链式反应(PCR)法对一株多重耐药铜绿假单胞菌进行β-内酰胺酶基因、氨基糖苷类修饰酶基因、氯霉素类基因、耐消毒剂和磺胺类基因检测。结果:PCR结果显示该株PA的TEM,MIR,CTX-M1群,OXA-1群,OXA-10群,aac(6′)-b,aac(6′)-,aac(3)-,ant(2″)-,catB,qacE△1-sul1基因均为阳性,GES,DHA,VEB,cmlA基因均阴性,Opr D2未缺失。结论:该株铜绿假单胞菌存在多重耐药基因,而且对胺类、胍类消毒剂耐受。
Objective：To study the instance and mechanism of multi-drug resistance of pseudomonas aeruginosa.Methods：The genes of multi-drug resistance including beta-lactamase,aminoglycosides modifying enzymes,chloramphenicol,disinfect resistance and sulfanilamide-resistance were determined by polymerase chain reaction（PCR）.Results：The results of PCR indicated that TEM,MIR,CTX-M1,OXA-1,OXA-10,aac（6′）-Ⅰb,aac（6′）-Ⅰ,aac（3）-Ⅱ,ant（2″）-Ⅰ,catB,qacE △1-sul1 genes were positive,while GES,DHA-2,VEB,cmlA genes were negative,Opr D2 is not missing.Conclusion：There are multi-drug resistance genes in the strain of pseudomonas aeruginosa,which resist disinfectant of amines and guanidines.
出处
《临床医药实践》
2011年第2期126-128,共3页
Proceeding of Clinical Medicine
基金
山西省卫生厅科技攻关计划项目
