摘要
目的:建立梭果黄芪药材中毛蕊异黄酮葡萄糖苷和芒柄花素含量测定方法,为完善梭果黄芪的质量标准提供依据。方法:Agilent TC-C18(4.6 mm×250 mm,5μm)色谱柱;以乙腈为流动相A,以水(含0.2%甲酸)为流动相B,梯度洗脱;检测波长260 nm;柱温30℃;流速1.0 mL/min;进样量10μL。结果:毛蕊异黄酮葡萄糖苷和芒柄花素的线性回归方程分别为Y=30.025 X+5.1096(r=0.999 7)和Y=58.24 X-94.279(r=0.999 7);线性范围分别为5.52～110.40μg/mL和5.54～110.72μg/mL;样品中毛蕊异黄酮葡萄糖苷和芒柄花素的总量在0.0053%～0.0179%之间。结论:本实验建立的毛蕊异黄酮葡萄糖苷和芒柄花素的含量测定方法简便、准确,可用于梭果黄芪药材中黄酮类成分的质量控制。
Objective：To establish the quantitative methods for calycosin-7-O-β-D-glycoside and formononetin in Astragalus ernestii Comb,in order to consummate a quality standards for medicinal Astragalus ernestii Comb.Methods：The HPLC condition was as follows：column：Agilent TC-C18 250 mm×（4.6 mm,5 μm）,mobile phase： A was ACN,B was H2O-0.2 %HCOOH,with gradient elution;flow speed：1.0 mL/min;detection：260 nm;temperature of column：30 ℃;injection volume：10 μL.Results：The calibration curves were linear within the range of 5.52～110.40 μg/mL for calycosin-7-O-β-D-glycoside and 5.54～110.72 μg/mL for formononetin,with their regression function being Y=30.025 X＋5.1096（r=0.999 7）and Y=58.24 X-94.279（r=0.999 7）;The content s of calycosin-7-O-β-D-glycoside and formononetin in Astragalus ernestii Comb were 0.0053%~0.0179%.Conclusion：HPLC is a simple and accuracy method for determining the contents of calycosin-7-O-β-D-glycoside and formononetin in Astragalus ernestii Comb.
出处
《成都中医药大学学报》
2010年第4期65-67,共3页
Journal of Chengdu University of Traditional Chinese Medicine
基金
四川省中药材标准起草说明项目(编号:川食药监注[2008]21号)
关键词
梭果黄芪
毛蕊异黄酮葡萄糖苷
芒柄花素
含量测定
Astragalus ernestii Comb
calycosin-7-O-β-D-glycoside
formononetin
Determination
