摘要
目的 对血清CK-MB活性大于总CK活性的现象进行原因初探及处理.方法 在HITACHI 7600-020全自动生化仪上采用免疫抑制法测定样本CK和CK-MB活性,依据CK-MB活性测定值分为恶性肿瘤患者组、心肌梗死组和正常组.同时利用热失活（将血清置于45℃水浴20 min后）再次测定其CK和CK-MB活性：在MINIVIDAS全自动荧光酶标仪上采用荧光免疫法测定CK-MB质量.结果 20例恶性肿瘤患者血清免疫抑制法测定CK-MB＞CK,热失活处理后CK-MB常规方法测定活性保存率在43.4%～78.1%,均数为59.2%;而心肌梗死组和健康体检者组的活性保存率均＜1%.结论 恶性肿瘤中的异常CK同工酶被认为是造成CK-MB假性增高的重要原因之一,利用热失活方法可以有效鉴别.CK-MB质量测定法因其方法学特性,可以排除上述干扰,是鉴别CK-MB假性增高的最佳方法.
Objective To study the special phenomena that CK-MB activity widely increases and may be even over the levels of total CK activity in the serum of some tumor patients. And explore the cause of this false increase of CK-MB activity by testing quantity of CK-MB and analysing its activity changes before and after thermal inactivation. Methods The CK and CK-MB activity of samples were detected by immunosuppression assay with HITACHI 7600-020 automatic biochemical analyzer. The samples were divided into three groups as CK-MB false increase group,myocardial infarction group and the normal control group based on CK-MB activity. At the same time,the CK and CK-MB activity were remeasured by thermal inactivation （serum handled by placing in 45 ℃ water and bathing for 20 minutes） assay and the CK-MB quantity was detected by fluorescence immunoassay with MINIVIDAS automatic fluorescence microplate reader. Results In tumor group, the range of CK-MB activity conservation rate was from 43.4% to 78.10% with the average of 59.2%. Compared to the tumor group,the CK-MB activity conservation rate in myocardial infarction group and control group were both less than 10%. Conclusion Thermal inactivation assay could be used effectively to discriminate the fake increase of the CK-MB activity,and can also be used to prove a real increase. The CK-MB quantity measuring assay cou＇uld eliminate the interference ,which is the best way to identify the false increase of CK-MB. Emerging of abnormal CK isoenzyme in malignancies is considered one of the most important reasons of CK-MB false increase.
出处
《现代检验医学杂志》
CAS
2010年第6期78-79,82,共3页
Journal of Modern Laboratory Medicine
