摘要
以春兰‘宋梅’为试验材料,研究了植物生长调节物质及有机添加物对春兰根状茎的增殖与分化的影响,结果表明适合春兰根状茎增殖的培养基为：1/2MS＋3~5 mg/L NAA＋0.1 mg/L BA＋10%椰子汁＋30 g/L蔗糖＋6.8 g/L琼脂;最适合根状茎芽分化的培养基为：1/2MS＋0.5 mg/L NAA＋3 mg/L BA＋10%椰子汁＋30 g/L蔗糖＋6.8 g/L琼脂。
A study was conducted to determine the effects of plant growth regulators（BA,NAA） and organic additives on the growth,proliferation and differentiation of the rhizomes of Cymbidium goeringii in vitro.Results showed that the optimum culture medium for proliferation of C.forrestii ＇Songmae＇ rhizomes was 12MS medium supplemented with 3-5 mgL NAA,0.1 mgL BA,10% coconut milk,30 gL sucrose,and 6.8 gL agar.12MS medium containing 0.5 mgL NAA,3 mgL BA,10% coconut milk,30 gL sucrose,and 6.8 gL agar was suitable for differentiation of C.forrestii ＇Songmae＇ rhizomes.
出处
《东北林业大学学报》
CAS
CSCD
北大核心
2010年第12期40-42,共3页
Journal of Northeast Forestry University
基金
浙江省中青年学科带头人培养资助项目(2272000004)
浙江省温州科技局国际合作项目(H20080049)
浙江省新苗人才计划项目(2008R40G2100075)
浙江省农业科学院国际合作专项经费资助项目
关键词
春兰
植物生长调节物质
有机添加物
根状茎
分化
Cymbidium goeringii
Plant growth regulators
Organic additives
Rhizomes
Differentiation
