摘要
本文采用杂交瘤技术，制备了７ 株抗甲型副伤寒沙门氏菌鞭毛抗原单克隆抗体（ｍ Ａｂ） 的杂交瘤细胞株。对３ 株ｍＡｂ（２Ｃ８、２Ｅ６ 和５Ｄ７） 进行了鉴定，ｍＡｂ ２Ｃ８ 和５Ｄ７ 为ＩｇＭ，２Ｅ６ 为ＩｇＧ１（κ）亚类，可分别识别不同的抗原表位，均具有很强的特异性，即只与甲型副伤寒沙门氏菌及其鞭毛抗原起反应，而与相关肠道细菌：尼亚里木沙门氏菌、达卡沙门氏菌及伤寒沙门氏菌、乙型和丙型副伤寒杆菌、猪霍乱沙门氏菌、纽波特沙门氏菌等均无交叉反应。免疫印迹显示，３ 株ｍＡｂ 只能与甲型副伤寒沙门氏菌鞭毛抗原Ｍｒ 为５２ ０００ 的蛋白结合。用ｍＡｂ ２Ｅ６ 和５Ｄ７ 建立的双ｍＡｂ 夹心ＥＬＩＳＡ 法，检测了１３ 例血培养阳性的副伤寒患者血清中的甲型副伤寒沙门氏菌鞭毛抗原，１２ 例呈阳性，阳性符合率为９２－３％ 。
Seven hybridma cell lines secreting monoclonal antibodies(mAb) to Salmonella paratyphi A flagellin protein were established by hybridma technique and three mAbs of them were characterized The results showed that mAb 2C8 and 5D7 were IgM(κ),mAb 2E6 was IgG(κ) They may recognize different epitopes on the flagellin protien and reacted only with S para typhi A and its flagellin protein, but not with other related enterobacteria including S niarem be, S dakar, S paralyphi B and C, S cholerae suis and S newport etc Immunoblotting ana lysis showed that 3 mAbs bound only to S papatyphi A flagellin protein of relative molecular mass(Mr) 52 000 A double mAb sandwich ELISA employing mAb 2E6(as coating) and 5D7(as enzyme labelled antibody) was used to detect S paratyphi A flagellin antigen in 13 samples from the patients with S paratyphi which blood culture was positive Twelve samples of them were positive and the coincidental rate was of 92 3%
出处
《细胞与分子免疫学杂志》
CAS
CSCD
1999年第4期300-301,323,共3页
Chinese Journal of Cellular and Molecular Immunology
