摘要
目的:探讨原发性高血压(EH)患者外周血单个核细胞(PBMC)炎症细胞因子-肿瘤坏死因子α(TNF-α)、白细胞介素6(IL-6)水平的变化。方法:采用密度梯度离心法分离30例EH患者(Ⅰ级9、Ⅱ级15、Ⅲ级6)PBMC,并用放射免疫分析法(RIA)检测PBMC分泌TNF-α、IL-6的含量,20名年龄与性别相匹配的健康者作为正常对照组。结果:EH患者PBMC分泌TNF-α、IL-6水平(178.4±49.4 ng/L、143.7±37.2 ng/L)明显高于正常对照组(109.5±27.3 ng/L、89.7±21.3 ng/L)(P<0.01)。结论:EH患者PBMC处于激活状态,存在炎症反应,可能参与原发性高血压的发生发展。
Objective：To investigate the effects of inflammatory cytokine-tumor necrosis factor-alpha（TNF-α） and interleukin-6（IL-6） in peripheral blood mononuclear cells（PBMC） from essential hypertensive（EH） patients.Methods：The levels of TNF-α and IL-6 IL-1β in PBMC from 30 cases of EH were measured by radio-immunologic assay（RIA）,20 age-and sex-matched healthy subjects served as controls.Results： The TNF-α and IL-6 levels（178.4±49.4 ng/L,143.7±37.2 ng/L） of PBMC in EH patients significantly higher than normal controls（109.5±27.3 ng/L,89.7±21.3 ng/L）（P0.01）.Conclusion：EH is associated with increased PBMC activation and may be involved in the pathogenesis of EH.
出处
《中国卫生检验杂志》
CAS
2010年第12期3358-3359,共2页
Chinese Journal of Health Laboratory Technology
